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Despite active lipolysis, re-esterification of fatty acids within adipocytes will prevent release of fatty acids into the bloodstream. Therefore, a measurement of fatty acid flux can underestimate the rate of lipolysis except under fasting conditions. However, glycerol cannot be reincorporated into triglycerides because glycerol kinase is absent in adipocytes. We use the rate of appearance of glycerol as a direct index of lipolysis.
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