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Intracellular recycling of fatty acids results when triglycerides are hydrolyzed within adipocytes and fatty acids are re-esterified instead of released into the blood stream. Since three fatty acids are released per glycerol molecule released, the intracellular recycling rate is equal to 3 times the flux of glycerol minus the free fatty acid flux. Extracellular recycling occurs when free fatty acids are released into the bloodstream and eventually re-esterified. The extracellular recycling rate is calculated as the free fatty acid flux minus the total fat oxidation. We use simultaneous isotopic infusions of labeled fatty acid and glycerol tracers to provide an index of the relative rate of fatty acid reesterification.
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