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 If all you see are spots you need to have the CNRU Mass Spectrometry Core do your advanced research for you.
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Lean and Fat Body Mass Determination Using CO2 EnrichmentThe CNRU Mass Spectrometry Core Facility offers assistance in methods development and mass spectrometry analysis to help researchers determine total body water using 18O-labeled water.Total Body Water (body composition) has been measured using stable isotopes of oxygen (18-Oxygen) and deuterium (deuterium oxide, D2O) for more than 40 years. It is a very useful technique to estimate lean body mass because it is easy to administer, noninvasive, accurate, and can be used in free-living subjects. The principle is based on the theory that water is distributed in all parts of the body except body fat. In a typical Total Body Water study, subjects fast for at least 2 hours and a "pre" baseline plasma sample is collected. Heavy water is precisely weighed out and administered orally or intravenously. A second sample is collected four hours later after complete equilibrium has been reached. The 18O content of aqueous fluid is measured by equilibration of the fluid with CO2 of known 18-O content. The following reaction occurs: H218O + C16O2 --> H216O + C16O18O The resulting CO2 gas can be isolated and purified for isotope ratio measurement. Total body water values are then converted to kilogram amounts and lean and fat body mass can be determined. Click for more detailed information about the calculations used to determine total body water, lean body mass and fat mass. |
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