Mass Spectrometry Lab UCDHSC Anesthesiology Research - Clinical Nutrition Research Unit
Difference_In_Gel_Electrophoresis
The CNRU Mass Spectrometry Core Facility is equipped for advanced Difference In Gel Electrophoresis (DIGE) experiments.

ENRICHING THE MEMBRANE FRACTION

Kits are available from BioRad (Membrane I and Membrane II) and Pierce for isolating and enriching membrane proteins. Please follow the links to the protocols. Before using the protocols you will generally:
  1. Pellet cells at 500 x g for 3 min. (Make sure you pre-weigh your tubes so you can determine a cell weight).
  2. Remove supernatant and wash 3-4 times in wash buffer. Remove all supernatant and re-weigh cells to obtain a wet weight.


Click here for recipes for our standard lysis buffers.
BioRad Membrane I proteins (.pdf)
BioRad Membrane II Proteins (.pdf)
Pierce Membrane Proteins (.pdf)
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