Mass Spectrometry Lab UCDHSC Anesthesiology Research - Clinical Nutrition Research Unit
Advanced_Clinical_Research
If all you see are spots you need to have the CNRU Mass Spectrometry Core do your advanced research for you.

ISOLATING THE MITOCHONDRIA

Kits are available from Sigma and Pierce for isolating and enriching mitochondrial proteins from both tissue and cell culture systems. Please follow the links to the protocols. Before using the protocols you will generally:
  1. Pellet cells at 500 x g for 3 min. (Make sure you pre-weigh your tubes so you can determine a cell weight).
  2. Remove supernatant and wash 3-4 times in wash buffer. Remove all supernatant and re-weigh cells to obtain a wet weight.
Click here for recipes for our standard lysis buffers.
Sigma_MitochondriaI_isolation.pdf
Pierce_Mitochondria_Tissue.pdf
Pierce_Mitochondria_Cell_Culture.pdf
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