A GENERAL SAMPLE PREPARATION WORKFLOW STRATEGY FOR 2DGE

Define your goals. What proteins do you want to see?
Simplify your sample based on your goals

Simplify The Sample

Check your conditions.
Do you see the proteins you anticipated?
Are the lysis conditions appropriate?

Re-evaluate.
Have you defined the proteins you're looking for?

Scale up using a single pH 3-10 or 4-7 strip and large gel.
Determine your upper and lower loading limits
and decide on the stain to use.