Mass Spectrometry Lab UCDHSC Anesthesiology Research - Clinical Nutrition Research Unit
Difference_In_Gel_Electrophoresis
The CNRU Mass Spectrometry Core Facility is equipped for advanced Difference In Gel Electrophoresis (DIGE) experiments.

PLASMA SAMPLE SIMPLIFICATION - IMMUNODEPLETION

High abundance proteins such as albumin, IgG, anti-trypsin, transferring and haptoglobin account for approximately 95% of the protein content in plasma. There are an estimated 20,000 remaining low abundance proteins present in plasma with a wide range of concentrations. The high abundance proteins are typically removed for best results in analyzing the low abundance proteins.

The CNRU Mass Spectrometry Core Facility employs several different methods for depleting plasma samples. Follow the links for more information.

PIERCE

ProteoSeek Albumin/IgG removal kit (#89875)
Cibacron blue dye non-specifically binds albumin/IgG

ProteoSeek Albumin/IgG removal kit (#89876)
Antibody-based removal

AGILENT

Multiple Affinity column-based removal (Immunodepletion). Depletes albumin, IgG, anti-trypsin, IgA, transferrin, and haptoglobin.
Pierce_Albumin-IgG_Removal_Kit.pdf
Pierce_Antibody-based_Albumin_Removal_Kit.pdf
Agilent_Multiple_Affinity_Removal_System.pdf
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