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Quantitative Small Molecule and Hormone Analysis for Pharmacological Research
The CNRU Mass Spectrometry Core Facility offers assistance in methods development and quantitative mass spectrometry analysis to help researchers assay for small molecules and hormones.
Ongoing assay developments include the development and validation of automated high-throughput assays for steroid hormones, markers of oxidative stress and endothelial dysfunction, catecholamines and GI peptide hormones in plasma. We have also concentrated in-house on the development of assays to profile lipid patterns and to quantify known compounds in these patterns. Thus, we developed an automated LC/LC-MS/MS assay for isoprostane patterns that also allowed for the quantification of 8-epi prostaglandin F2α with high specificity and sensitivity. At present, we employ GC-MS, LC-MS and LC-MS/MS for the quantification of small molecules. We primarily use Applied Biosystems API 4000 and 5000 triple quadrupole mass spectrometers for these assays.
Our faculty is very experienced in the quantification of drugs, their metabolites and endogenous compounds in body fluids, tissues, cell cultures, and sub-cellular components such as mitochondria, providing the expertise to rapidly develop highly specific assays for your compounds.
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