Evolutionary conservation

of the active core of CBS and human mutations

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                       Ex1|                                Ex2|    
                                                          Q 
                                  R      R NS            PN      R    V R 
CBS HS        61 SESPHHHTAPAKSPKILPDILKKIGDTPMVRINKIGKKFGLKCELLAKCEFFNAGGSVKD 120
CBS SC        14 -------------------VIDLVGNTPLIALKKLPKALGIKPQIYAKLELYNPGGSIKD 54
CBS RN        58 ADSPHYHTVPTKSPKILPDILRKIGNTPMVRINRISKNAGLKCELLAKCEFFNAGGSVKD 117
CYSK EC       01 ----------------MSTLEQTIGNTPLVKLQRMGPDN--GSEVWLKLEGNNPAGSVKD 42
CYSK ST       01 -------------SKIYEDNSLTIGHTPLVRLNRIGNG-----RILAKVESRNPSFSVKC 42
CSYN SO       01 --------MVEEKAFIAKDVTELIGKTPLVYLNTVADGC--VARVAAKLEGMEPCSSVKD 50

                 :.:.   :   ...         :*.**:: :: :    .   .:  * *  :.  *:* 



                 L   P
                 H   W                       Ex3|                     Ex4|
                 C   QV D  D       R    MKL  R  RMR T P       Y  M       K
CBS HS       121 RISLRMIEDAERDGTLKPG-DTIIEPTSGNTGIGLALAAAVRGYRCIIVMPEKMSSEKVD 179
CBS SC        55 RIAKSMVEEAEASGRIHPSRSTLIEPTSGNTGIGLALIGAIKGYRTIITLPEKMSNEKVS 114
CBS RN       118 RISLRMIEDAERAGTLKPG-DTIIEPTSGNTGIGLALAAAVKGYRCIIVMPEKMSMEKVD 176
CYSK EC       43 RAALSMIVEAEKRGEIKPG-DVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRA 101
CYSK ST       43 RIGANMIWDAEKRGVLKPG-VELVEPTNGNTGIALAYVAAARGYKLTLTMPETMSIERRK 101
CSYN SO       51 RIGFSMITDAEKSGLITPGESVLIEPTSGNTGIGLAFIAAAKGYKLIITMPASMSLERRT 110
                 * .  *: :**  * : *.   ::*.*.*****.**  .* :**:  : :* .** *:  



                                                         Ex5|     
                                                                  S 
                 A          M      V                          H T K  LD N
CBS HS       180 VLRALGAEIVRTPTNARFDSPESHVGVAWRLKNEIPNSH-ILDQYRNASNPLAHYDTTAD 238
CBS SC       115 VLKALGAEIIRTPTAAAWDSPESHIGVAKKLEKEIPGAV-ILDQYNNMMNPEAHYFGTGR 173
CBS RN       177 VLRALGAEIVRTPTNARFDSPESHVGVAWRLKNEIPNSH-ILDQYRNASNPLAHYDDTAE 235
CYSK EC      102 AMRAYGAELILVTKEQGMEG---ARDLALEMANRGEGKL--LDQFNNPDNPYAHYTTTGP 156
CYSK ST      102 LLKALGANLVLTEGAKGMKG---AIQKAEEIVASDPQKYLLLQQFSNPANPEIHEKTTGP 158
CSYN SO      111 ILRAFGAELILTDPAKGMKG---AVQKAEEIRDKTPNSY-ILQQFENPANPKVHYETTGP 166
                  ::* **::: .      ....:    * .:          *:*: *  **  *   *. 



                    Ex6|                     	           Ex7|
                                          D  R   G           
                 K   X                  M S  M   K     del       Y  T            L
CBS HS       239 EILQQCDG-----KLDMLVASVGTGGTITGIARKLK--EKCPGCRIIGVDP-EGSILAEP 290
CBS SC       174 EIQRQLEDLNLFDNLRAVVAGAGTGGTISGISKYLK--EQNDKIQIVGADP-FGSILAQP 230
CBS RN       236 EILQQCDG-----KVDMLVASAGTGGTITGIARKLK--EKCPGCKIIGVDP-EGSILAEP 287
CYSK EC      157 EIWQQTGG-----RITHFVSSMGTTGTITGVSRFMR--EQSKPVTIVGLQPEEGSS---- 205
CYSK ST      159 EIWEDTDG-----QVDVFISGVGTGGTLTGVTRYIKGTKGKTDLITVAVEPTDSPVIAQA 213
CSYN SO      167 EIWKGTGG-----KIDIFVSGIGTGGTITGAGKYLK--EQNPDVKLIGLEPVESAVLSGG 219

                 ** .   .     .:  .::. ** **::*  : ::  :       :. :*  ..  :  



                                         Ex8|                        Ex9|
                                                         E    H                     
                            K  R S            A          V    C P        S N
CBS HS       291 EELNQTEQTTYEVEGIGYDFIPTVLDRTVVDKWFKSNDEEAFTFARMLIAQEGLLCGGSA 350
CBS SC       231 ENLNKTDITDYKVEGIGYDFVPQVLDRKLIDVWYKTDDKPSFKYARQLISNEGVLVGGSS 290
CBS RN       288 EELNQTEQTAYEVEGIGYDFIPTVLDRAVVDRWFKSNDDDSFAFARMLISQEGLLCGGSS 347
CYSK EC      206 ------IPG---IRRWPTEYLPGIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSS 256
CYSK ST      214 LAGEEIKPGPHKIQGIGAGFIPGNLDLKLIDKVVGITNEEAISTARRLMEEEGILAGISS 273
CSYN SO      220 ------KPGPHKIQGLGAGFIPGVLDVNIIDEVVQISSEESIEMAKLLALKEGLLVGISS 273
                    ..      .:.     ::*  ::  ::*      .  :    : *  .**:: * *:


                                              Ex10|                      Ex11| 
                                      C              N
                  NMMP     T          HYM    N  Q    E     XI
CBS HS       351 GSTVAVAVKAAQ---ELQEGQRCVVILPDSVRNYMTKFLSDRWMLQKGFLKEEDLTEKKP 407
CBS SC       291 GSAFTAVVKYCEDHPELTEDDVIVAIFPDSIRSYLTKFVDDEWLKKNNLWDDDVLARFDS 350
CBS RN       348 GSAMAVAVKAAQ---ELKEGQRCVVILPDSVRNYMSKFLSDKWMLQKGFMK-EELSVKRP 403
CYSK EC      257 GGAVAGALRVAK----ANPDAVVVAIICDRGDRYLSTGVFGEEHFSQGAGI--------- 303
CYSK ST      274 GAAVAAALKLQED--ESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ--------- 322
CSYN SO      274 GAAAAAAIKVAKR--PENAGKLIVAVFPSFGERYLSSVLFDSVRKEAESMVIES------ 325
                 *.: : .::  :       .   *.:: .    *::. :      .      .  :   .

Sequences:

CBS HS, human CBS (Homo sapiens);
CBS SC, yeast CBS (Saccharomyces cerevisiae);
CYSK RN, rat CBS (Rat (form IV));
CYSK EC, E.coli O-acetylserine lyase (Escherichia coli);
CYSK ST, Salmonella typhimurium O-acetylserine lyase (Salmonella typhimurium);
CYSN SO, spinach cysteine synthase (Spinacia oleracea).

Aligned by CLUSTALW 1.82.