David J. Orlicky
Assistant Professor
Ph.D., University of Colorado Denver, 1984


david.orlicky@uchsc.edu
 

Teaching: I teach the graduate student histology course, Pathology 7620, entitled Normal and Abnormal Biological Structures. I also help teach the pathology graduate student journal club, Pathology 7613.

Research: A majority of my research revolves around a protein called FPRP. This is a protein that I discovered, purified, and cloned the cDNA for. FPRP is a single pass transmembrane protein whose structure includes immunoglobulin loops much as one finds in receptor-type proteins. FPRP appears to play an essential role in the accumulation of triacylglycerol in adipocytes. It is also found in cardiomyocytes, skeletal muscle cells, granulosa lutein cells, leydig cells, and a few other cell types. Current experiments are directed at discerning the protein's biochemical function and how it is regulated within these cell types. Obesity is a major cause of morbidity and mortality; it predisposes to both diabetes and hypertension which themselves predispose to cardiovascular disease. In this modern day of relative physical inactivity, obesity is considered by many to be the leading health problem facing the developed world. Drs. Jerry Schaack, Department of Microbiology, UCD and Jim McManaman, Department of Physiology, UCD collaborate with me on this project.

In addition, I also work with Jim on his research examining the relationship of ADPH and Perilipin to lipid accumulation.

Relevant Publications:

  1. Orlicky, D.J., and Nordeen, S.K. Cloning, sequencing and proposed structure for a prostaglandin F2a receptor regulatory protein. Prostaglandins, Leukotrienes and Essential Fatty Acids. 55:261-268, (1996).
  2. Orlicky, D.J. Negative regulatory activity of a prostaglandin F2a receptor associated protein (FPRP). Prostaglandins, Leukotrienes and Essential Fatty Acids. 54:247-259, (1996).
  3. Orlicky, D.J., Lieber, J.G., Morin, C.L., and Evans, R.M. Synthesis and accumulation of a receptor regulatory protein associated with lipid droplet accumulation in 3T3-L1 cells. J Lipid Research 39:1152-1161, 1998.
  4. Orlicky, D. J. and Schaack, J. Adenovirus Transduction of 3T3-L1 cells. J Lipid Res. 42:460-6, 2001.
    Stipp, C. S., Orlicky, D. J., and Hemler, M. E. FPRP: A major, highly stoichiometric, highly specific CD81 and CD9-associated protein. J Biol Chem. 276:4853-4862, 2001.
  5. Orlicky, D. J., DeGregori, J., and Schaack, J. Construction of Stable Coxsackievirus and Adenovirus Receptor Expressing 3T3-L1 Cells. J Lipid Res 42:910-5, 2001.
  6. Schaack, J, Allen, B, Orlicky, DJ, Bennett, M, Maxwell, IH, Smith, RL. Promoter Strength in Adenovirus Transducing Vectors: Down-Regulation of the Adenovirus E1A Promoter in 293 Cells Facilitates Vector Construction. Virology 291:101-109, 2001.
  7. Lehner R, Lucia MS, Jarboe EA, Orlicky D, Shroyer AL, McGregor JA, Shroyer KR. Immunohistochemical localization of the IAP protein survivin in bladder mucosa and transitional cell carcinoma. Appl Immunohistochem Mol Morphol 10:134-8, 2002.
  8. Frost, M, Jarboe, EA, Orlicky, D, Gianani, R, Thompson, LC, Enomoto, T, Shroyer, KR. Immunohistochemical localization of survivin in benign cervical mucosa, cervical dysplasia, and invasive squamous cell carcinoma. Am J Clin Pathol 117:738-44, 2002.
  9. McManaman, JL, Zabaronick, W, Schaack, J, Orlicky, DJ. Lipid Droplet Targeting Domains of Adipophilin. J Lipid Res, 44:668-673, 2003.
  10. Ross, SA, Song, X, Burney, MW, Kasai, Y, Orlicky, DJ. Efficient Adenovirus Transduction of 3T3-L1 Adipocytes Stably Expressing Coxsackie-Adenovirus Receptor. Biochem Biophys Res Commun. 302:354-8, 2003.
  11. Liggins, C, Orlicky, DJ, Bloomquist, LA, Gianani, R. Developmentally regulated expression of surviving in human islets. Accepted for publication, Human Pathology, June 2003.

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