Reagents
Mouse models
CT-TCR: Trangenic mouse expressing TCR specific for the AH1/H-2Ld complex from the CMV promoter and Ig enhancer. Low expression in BALB/c mice (back-crossed 24 generations). Detectable expression on a Rag KO background as described in Jordan 2008. Many coreceptor negative, CD3 positive T cells in the periphery and thymus.
gp70 KO: Congenic mouse with BALB/c Chr 5 Mb 28-32 (encoding the MuLV that harbors the tumor antigen gp70) replaced with C57BL/6 interval which does not have gp70 in this region. The details of the mouse construction are available in McWilliams 2008. 107 SNPs reveal BALB/c loci after 5 back-crosses, the strain has been further back-crossed to BALB/c 25 generations.
