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Olympus IX81 inverted motorized microscope with spinning disk attachment. Room 7402C.
How does it work?
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DETAILS:
OLYMPUS IX81 inverted motorized Microscope. Olympus objectives- U PLAN APO 10X oil N.A. 0.40, UAPO 40X oil N.A. 1.35, U PLAN APO 100X oil N.A. 1.35. EXCITATION SOURCE: 100 watt Hg lamp. CAMERA; Hamamatsu ORCA IIER monochromatic CCD camera 1344X1024 full chip, 6.45X6.45 um pixel . FILTER SETS: DAPI EX360-70 EM420-60, FITC EX450-80 EM535, TRITC EX535 EM635; CFP/YFP FRET filter set 8600 2v2(JP4)
PLATFORM: Dell P4 3.2 GHz, Windows 2000 . DATA: ethernet, CD-W or DVD-W 16X. Acquisition software: Intelligent Imaging Slidebook v.4.067.
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Olympus Total Internal Reflection Fluorescence (TIRF) microscope. Room 7402A1.
What is TIRF?
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DETAILS:
OLYMPUS IX81 inverted motorized Microscope, Prior motorized XY stage, Environmental Chamber. Olympus objectives- U PLAN Fluorite 10X N.A. 0.30 WD 10mm, LWD PLAN Fluorite 40X N.A. 0.60 WD 2.89-2.15 mm, TIRFM PLAN APO 60X oil N.A. 1.45 WD 0.15mm. EXCITATION SOURCE: Widefield fluorescence- 100 watt Hg lamp, TIRF laser illumination- Argon 10mW 488nm; 543 HeNe, 633 HeNe . CAMERA; Hamamatsu ORCA ER monochromatic CCD camera 1344X1024 full chip, 6.45X6.45 um pixel .
FILTER SETS : Widefield Fluorescence- FITC EX480/40 EM 535/50, TRITC EX535/50 EM610/75 , Dual Band FITC/TRITC TIRF filters: 488 TIRFM Filter Set:Z-488\10 DMZ500RDC 515/50M , 543 TIRFM Filter Set Z-543\10 DMZ543RDC 590/50M, 633 TIRFM Filter Set Z-633\10 DMZ633RDC 700/75M
PLATFORM: Dell P4 3.2 GHz, Windows 2000 . DATA: ethernet, CD-W or DVD-W 16X. Acquisition software: Intelligent Imaging Slidebook v.4.067.
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Deltavision digital deconvolution rig. Inverted microscope with computer-controlled stage. Chilled CCD camera. Advanced software. Room 7402B.
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DETAILS: OLYMPUS IX70 Infinity Microscope (Inverted). Computer-controlled stage (x,y,z axes) allowing multiple point time lapse and z-axis sectioning. Stage heater. Olympus objectives: 100X U PLAN APO, N.A. 1.35, W.D. 0.1 mm, oil; 60XW U PLAN APO, N.A. 1.20, W.D. 0.25 mm, water; 20X locator lens. Separate excitation and emission filter wheels. Excitation: 100 Watt Hg arc lamp; Camera: Photometrics Quantix cooled CCD camera with KAF 1400 chip (1024X1024 max field, 6.7x6.7 um pixel physical size). Filter sets: DAPI (Ex 360/40, Em 457/50); FITC (Ex 490/20, Em 528/38);
RHOD/TR (Ex 555/28, Em 617/73); CY5 (Ex 640/20, Em 685/40). Dichroic: Chroma Quad Beamsplitter. 1.5x Optivar. Platform: SGI O2 R5000 180MHz processor, IRIX 6.3. DATA: Hard disc, Ethernet. Software: Deltavision digital deconvolution using empirically measured PSFs.
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| Zeiss two photon microscope. Zeiss LSM 510 NLO laser scanning confocal microscope with META detector for capturing emission spectra. Seven laser excitation lines, including tunable 2 photon infrared laser, plus brightfield (DIC). Room 7206.
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| DETAILS: Zeiss AXIOSKOP 2 FS MOT (Upright). Zeiss objectives: 5X Fluar, N.A. 0.25, W.D. 12.2 mm, dry; 10X Plan-Neofluar, N.A. 0.3, W.D. 5.6 mm, dry; 25X Plan-Neofluar, N.A. 0.8, W.D. 0.21 mm, oil/water; 40X Achroplan, N.A. 0.8, W.D. 3.6 mm, water; 63X IR Achroplan, N.A. 0.9, W.D. 2.0 mm, water; 63X Apochromat, N.A. 1.4, W.D. 0.09 mm, oil. Excitation: Lasers: Argon 458nm, 477nm, 488nm, 514nm; HeNe 543nm; HeNe 633nm; Coherent Mira Ti:Sapphire tunable IR laser (720-900 nm) for 2-photon excitation. 2 Non-descan detectors; 3 Descan detectors; 1 Transmitted light detector (DIC capable). Wide range of filter options with 3 PMT detectors. Platform: Siemens Pentium 4 1.7GHz processor (WINDOWS NT). DATA: CD-RW, Ethernet.
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| Lasers on the Zeiss LSM 510.
The Argon laser has four excitation lines (458, 477, 488, and 514 nm) and the two HeNe lasers offer additional lines at 543 nm and 643 nm. The IR 2 photon laser is a Coherent Mira 70 MHz mode locked Ti-Sapphire laser, tunable from ~720 nm to ~900 nm.
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Accessories
Printer and workstation: Fuji Pictography 3000 (320dpi RGB printer 8.5X11 or 4.25X11). Pentium 3 200MHz Windows 98.
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Workstations and software for image processing, archiving, and hard copy
A PC workstation is available for processing of images, with several different commercial and custom software programs. SGI workstations include two O2's for deconvolving and procesing. Data can be archived with Zip, Jazz, and CD media, as well as sent via ethernet to other computers.
Utility workstation
Microscope: Leitz Laborlux upright epifluorescence
Camera: Photometrics PXL KAF 1400 (high spatial resolution)
Computer: SGI Indigo (Inovision software)
Other lab equipment
A cell culture incubator is available for temporary storage of specimens during live cell imaging experiments. A dissecting microscope is available.
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