UCD DERC Histology & Flow Cytometry Core
Histology Core
Routine histological services in tissue fixation, processing, sectioning, and staining with histochemical dyes and immunochemical reagents. Provision of slide and tissue banks of commonly used tissues or animal models e.g. developmental or NOD disease progression.
Epifluorescence microscopy for acquisition of images and performance of morphometric analyses.
Confocal microscopy for use by investigators interested in high-resolution fluorescence microscopy and live cell imaging requiring the use of FRAP and FRET analyses.
In-situ hybridization analyses that include all elements from acquisition and amplification of the gene of interest, probe synthesis, optimization of hybridization conditions, and performance of multigene analyses on a moderate scale.
Higher-level microscopy including access to instrumentation and services in Laser Capture Microscopy, 2-photon confocal microscopy, spinning disk confocal microscopy, Total Internal Reflectance microscopy, conventional, and immunoelectron microscopy.
Training of fellows and students in morphological and morphometric techniques and consultation in specific areas including microdissection and single cell microinjection, low-level computer programming.
Contact personnel:
Roberto Gianani, MD and Antony Valentine
Flow Cytometry Core
Instrumentration
The Flow Cytometry Core lab has two cytometers available for researchers:
- BD FACSCalibur - good for up to 4-color acquisition
Blue laser (488nm)
Red laser (633nm)
- BD LSRII - good for up to 10-color acquisition
Red laser (633nm)
Blue laser (488nm)
Violet laser (405nm)
Ultraviolet laser (355nm)
The cytometers are maintained at the highest standard through the manufacturer’s (BD) preventative maintenance programs and by daily in-house Quality Control procedures.
Services
Cytometer Training - Since most researchers prefer to have their own staff run their samples, the Flow Cytometry Core lab will train your personnel to properly operate both our instruments.
Real-time sample collection assistance & trouble shooting - The Core’s manager is normally available to assist you in sample acquisition and analysis.
Technical Consultation - Dr. Dirk Homann (Cytometry Core Director) has made his expertise available to help resolve any cytometry-related problems - from experimental layout, cytochrome choices and sample preparation, through sample collection and analyses.
Contact personnel:
Phil Pratt and Dirk Homann, PhD
The purpose of this website is to provide some basic information about the DERC cores including contact information and links to on-line services where they are available. If you have questions about the UCD DERC please contact us (email or phone: 303.724.6836).
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