UCD DERC Molecular Biology Services
Tips for DNA sequencing
Sample Preparation:
- Automated sequencing is very sensitive to template and primer preparation.
- It is essential that the templates be free from ethanol, salts and other contaminants, as these greatly affect the quality of the sequencing results.
- Please note that phenol/chloroform extractions as a method of clean-up is not recommended as any residual phenol will inhibit the sequencing reaction.
Sample Submission:
To submit samples to the MBCF, first complete a sequencing request.
- Data Collection Only: samples must be provided as a dried pellet in 0.2 ml reaction tubes.
- Reaction Plus Data Collection: samples should be submitted in the following form:
- For plasmid DNA - provide 25 µL of DNA at a concentration of 20-35 ng/µL in water and for PCR products - provide 25 µL of DNA at a concentration of 5-20 ng/µL in water.
- The following standard primers are provided: M13F/pUCF, M13R/pUCR T7, SP6, T3, M13F(-20) and M13R(-27).
- For a custom primer, please provide 5 µL at a 10 µM concentration and include the Tm of the primer.
- DNA samples that are submitted for reaction plus data collection service must be accurately quantitated and prepared. Samples submitted with inaccurate concentrations and/or with contaminants present will result in poor sequence results or a failed reaction, which will still be billed to the user.
- After processing the results will be available to you from the webpage. Please login and click on “View your sequencing results.
- The sequence will be repeated at no extra cost if there is an operator error or machine malfunction. Please provide accurately quantitated, high quality DNA for successful results.
NOTE: WE WILL NOT REPEAT SAMPLES AT NO CHARGE IF THE DNA IS OF BAD QUALITY OR INACCURATE CONCENTRATION.
Links to Freeware for Chromatogram Viewing:
The purpose of this website is to provide some basic information about the DERC cores including contact information and links to on-line services where they are available. If you have questions about the UCD DERC please contact us (email or phone: 303.724.6836).
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