UCD DERC Molecular Biology Services
Automated DNA Sequencing
- Dye-terminator sequencing on ABI 3130xl and ABI 3100-Avant capillaries
- Online sample submission and results retrieval
- 24-36 hour turnaround time
- Next business day for samples submitted before 12pm
- See Sample Submission Guidelines for details and requirements
Automated Fragment Analysis
- Microsatellite analysis
- SNP detection
- Determination of STR or VNTR number
Real Time PCR
- Roche LC480 and AB 7000 SDS
- High Resolution Melt Capable
- Genotyping
- Gene Quantification and Expression analysis
in-situ hybridization probe synthesis
- Full scale hybridization from gene of interest selection to imaging and analysis
- Preparative work underway to build probe library for islet related targets (insulin, glucagon, slc30a8, etc)
- In conjunction with the DERC Histology Core
Basic Molecular Biology Services
- Nucleic acid purification
- Cloning and expression of genes of interest in bacterial systems
- Plasmid DNA preparation (small-large scale)
- Site directed mutagenesis
- Restriction digestion and analysis
- RT-PCR
- PCR and PCR product purification
- Recombinant protein expression and purification
Custom Services
The purpose of this website is to provide some basic information about the DERC cores including contact information and links to on-line services where they are available. If you have questions about the UCD DERC please contact us (email or phone: 303.724.6836).
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