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Molecular Biology

JEROME SCHAACK
Full Publications

Mao, J., Schaack, J., Sharp, S., Yamada, H., Kohli, J. and Söll, D. (1981). Transfer RNA and Nonsense Suppression in Schizosaccharomyces pombe. Molecular Genetics in Yeast, Alfred Benzon Symposium, 276-290 in Molecular Genetics of Yeast, Alfred Benzon Symposium 16. D. von Wettstein, J. Friis, M. Kielland-Brandt and A. Stenderup. Munksgaard, Copenhagen, Eds.

Mao, J., Appel, B., Schaack, J., Sharp, S., Yamada, H., and Söll, D. (1982). The 5S RNA Genes of Schizosaccaromyces pombe. Nucl. Acids Res. 10:487-500.

Schaack, J., Mao, J. and Söll, D. (1982). The 5.8S RNA Gene Sequence and the Ribosomal Repeat of Schizosaccharomyces pombe. Nucl. Acids Res. 10:2851-2864.

Dingermann, T., Burke, D.J., Sharp, S., Schaack, J., and Söll, D. (1982). The 5' Flanking Sequences of Drosophila tRNAArg Genes Control their in vitro Transcription in a Drosophila Cell Extract. J. Biol. Chem. 257:14738-14744.

Dingermann, T., Sharp, S., Schaack, J., DeFranco, D., Johnson, D., Cooley, L., and Söll, D. (1982). The in vitro Transcription of Drosophila tRNA Genes, pp. 219-233 in Gene Regulation, XXVI. B. O'Malley, ed., UCLA Symposia on Molecular and Cellular Biology.

Sharp, S., Dingermann, T., Schaack, J., and Söll, D. (1983). Transcription of Eukaryotic tRNA Genes in vitro: I. Analysis of Control Regions Using a Competition Assay. J. Biol. Chem. 258:2440-2446.

Schaack, J., Sharp, S., Dingermann, T., and Söll, D. (1983). Transcription of Eukaryotic tRNA Genes in vitro: II. Formation of Stable Complexes. J. Biol. Chem. 258:2447-2453.

Dingermann, T., Sharp, S., Schaack, J., and Söll, D. (1983). Stable Complex Formation of Eukaryotic tRNA Genes is Dependent on a Limited Separation of the Two Intragenic Control Regions. J. Biol. Chem. 258:10395-10402.

Burke, D.J., Schaack, J., Sharp, S., and Söll, D. (1983). Partial Purification of Drosophila Kc Cell Polymerase III Transcription Components: Evidence for Shared 5S RNA and tRNA Gene Factors. J. Biol. Chem. 258:15224-15231.

Sharp, S., Dingermann, T., Schaack, J., Sharp, J.A., Burke, D.J., DeRobertis, E.M., and Söll, D. (1983). Each Element of the Drosophila tRNAArg Gene Split Promoter Directs Transcription in Xenopus Oocytes. Nucl. Acids Res. 11:8677-8690.

Schaack, J., Sharp, S., Dingermann, T., Burke, D.J., Cooley, L., and Söll, D. (1984). The Extent of a Eukaryotic tRNA Gene: 5'- and 3'-Flanking Sequence Dependence for Transcription and Stable Complex Formation. J. Biol. Chem. 259:1461-1467.

Cooley, L., Schaack, J., Burke, D.J., Thomas, B., and Söll, D. (1984). Transcription Factor Binding is Limited by the 5'-Flanking Regions of a Drosophila tRNAHis Gene and a tRNAHis Pseudogene. Mol. Cell. Biol. 4:2714-2722.

Schaack, J. and Söll, D. (1985). Transcription of a Drosophila tRNAArg Gene in Yeast Extract: 5'-Flanking Sequence Dependence for Transcription in a Heterologous System. Nucl. Acids Res. 13:2803-2814.

Hottinger-Werlin, A., Schaack, J., Lapointe, J., Mao, J., Nichols, M., and Söll, D. (1985). Dimeric tRNA Gene Arrangement in Schizosaccharomyces pombe Allows Increased Expression of the Downstream Gene. Nucl. Acids Res. 13:8739-8747.

Sharp, S., Schaack, J., Cooley, L., Burke, D.J., and Söll, D. (1985). Structure and Transcription of Eukaryotic tRNA Genes. CRC Critical Reviews in Biochemistry 19:107-144.

Willis, I., Frendewey, D., Nichols, M., Hottinger-Werlin,A., Schaack, J., and Söll, D. (1986). A Single Base Change in the Intron of a Serine tRNA Affects the Rate of RNase P Cleavage in vitro and Suppressor Activity in vivo in Saccharomyces cerevisiae. J. Biol. Chem. 261:5878-5885.

Moore, M., Schaack, J., Baim, S.B., Morimoto, R.I., and Shenk, T. (1987) Induced Heat Shock mRNAs Escape the Nucleocytoplasmic Transport Block in Adenovirus-Infected HeLa Cells. Mol. Cell. Biol. 7:4505-4512.

Schaack, J. and Shenk, T. (1989) Adenovirus Terminal Protein Mediates Efficient and Timely Activation of Viral Transcription. Curr. Topics Microbiol. Immunol. 144:185-190.

Schaack, J., Schedl, P., and Shenk, T. (1990) Topoisomerase I and II Cleavage of Adenovirus DNA in vivo: Both Topoisomerase Activities appear to be Required for Adenovirus DNA Replictation. J. Virol. 64:78-85.

Schaack, J., Schedl, P., and Shenk, T. (1990) Transcription of Adenovirus and HeLa Cell Genes in the Presence of Drugs that Inhibit Topoisomerase I and II Function. Nucleic Acids Res. 18:1499-1508.

Schaack, J., Ho, W., Freimuth, P., and Shenk, T. (1990) Adenovirus Terminal Protein Mediates Nuclear Matrix Association and Efficient Transcription of Adenovirus DNA. Genes Devel. 4:1197-1208

Schaack, J., Logan, J., Vakalopoulou, E., and Shenk, T. (1991) Adenovirus E1A Protein Activates Transcription of the E1A Gene Subsequent to Transcription Complex Formation. J. Virol. 65:1687-1694.

Vakalopoulou, E., Schaack, J., and Shenk, T. (1991) A 32 kd Protein Binds to AU-Rich Domains in the 3' Untranslated Region of Rapidly Degraded mRNAs. Mol. Cell. Biol. 11:3355-3364.

Schaack, J., Langer, S., and Guo, X. (1995) Efficient Selection of Recombinant Adenoviruses using Vectors that Express ?-Galactosidase. J. Virol. 69:3920-3923.

Schaack, J., Guo, X., Ho, W. Y.-W., Karlok, M., Chen, C., and Ornelles, D. (1995) Adenovirus Type 5 Precursor Terminal Protein-Expressing 293 and HeLa Cell Lines. J. Virol. 69:4079-4085.

Schaack, J., Maguire, H., and Siddiqui, A. (1996) Hepatitis B Virus X Protein Partially Substitutes for E1A Transcriptional Function during Adenovirus Infection. Virology 216:425-430.

Langer, S. and Schaack, J. (1996) 293 Cell Lines that Inducibly Express Adenovirus Precursor Terminal Protein. Virol. 221:172-179.

Staley, K., Smith, R., Schaack, J., Wilcox, C., and Jentsch, T. (1996) Alteration of GABA-A Receptor Function Following Gene Transfer of the ClC-2 Chloride Channel. Neuron, 17:543-551.

Qadri, I., Conaway, J.W., Conaway, R.C., Schaack, J., and Siddiqui, A. (1996) Hepatitis B Virus Transactivator Protein, HBx, Associates with the Components of TFIIH and Stimulates the DNA Helicase Activity of TFII H. Proc. Natl. Acad. Sci. USA, 93:10578-10583.

Schaack, J., Guo, X., and Langer, S. (1996) Characterization of a Replication-Incompetent Adenovirus Type 5 Mutant Deleted for the Preterminal Protein Gene. Proc. Natl. Acad. Sci. USA, 93:14686-14691.

Kaddis, F.G., Zawada, W.M., Schaack, J., and Freed, C.R. (1996) Conditioned Medium from Aged Primate Fibroblasts Stably Expressing GDNF and BDNF Improve Survival of Embryonic Dopamine Neurons in vitro. Cell Tissue Res., 286:241-247.

Reed, D.S., Romero, P., Rimoldi, D., Cerottini, J.-C., Schaack, J., and Jongeneel, C.V. (1997) Construction and Characterization of a Recombinant Adenovirus Directing Expression of the MAGE-1 Tumor Specific Antigen. International J. Cancer, 72:1045-1055.

Maxwell, I.H., Maxwell, F., and Schaack, J. (1998) An Adenovirus Type 5 Mutant Deleted for the Preterminal Protein Gene efficiently Provides Helper Functions for Production of Recombinant Adeno-associated Virus Production. J. Virol. 72:8371-8373.

Leon, R.P., Hedlund, T., Meech, S.J., Li, S., Schaack, J., Hunger, S.P., Duke, R.C., and DeGregori, J. (1998) Adenoviral Mediated Gene Transfer in Lymphocytes. Proc. Natl. Acad. Sci. USA 95:13159-13164.

Moorhead, J.W., Clayton, G.H., Smith, R.L., and Schaack, J. (1999) A Replication-Incompetent Adenovirus Vector Deleted for the Preterminal Protein Gene Efficiently Transduces Mouse Ears. J. Virol. 73:1046-1053.

Hedlund, T.E., Meech, S.J., Srikanth, S., Kraft, A.S., Miller, G.J., Schaack, J., and Duke, R.C. (1999) Adenovirus-mediated Expression of Fas Ligand in Human Prostate Cancer Cell Lines. Cell Death and Differentiation 6:175-182.

Fagan, K.A., Rich, T.C., Tolman, S., Schaack, J., Karpen, J.W., and Cooper, D.M.F. (1999) Adenovirus-mediated Expression of an Olfactory Cyclic Nucleotide-gated Channel Regulates the Endogenous Ca2+-inhibitable Adenylyl Cyclase in C6-2B Glioma Cells. J. Biol. Chem. 274:12445-12453.

Schaack, J., Ho, W. Y.-W., Tolman, S., Ullyat, E., Guo, X., Frank, N., Freimuth, P.I., Roovers, J., and Sussenbach, J. (1999) Construction and Preliminary Characterization of a Library of "Lethal" Preterminal Protein Mutant Adenoviruses. J. Virol. 73:9599-9603.

Zhou, W., Hurlbert, M.S., Schaack, J., Prasad, K.N., and Freed, C.R. (2000) Overexpression of Human ?-Synuclein Causes Dopamine Neuron Death in Rat Primary Culture and Immortalized Mesencephalon-derived Cells. Brain Res. 866:33-43.

Rich, T.C., Fagan, K.A., Nakata, H., Schaack, J., Cooper, D.M.F., and Karpen, J.W. (2000) A High-Resolution, Single-Cell Measurement Reveals that cAMP is Produced in Microdomains. J. Gen. Physiol. 116:147-161.

Beeman, N., Nguyen, D.-A., Lewis, M., Schaack, J., and Neville, M.C. (2000) Intraductal Injection into the Mouse Mammary Gland. In Methods in Mammary Gland Biology and Breast Cancer Research, M.M. Ip and B.B. Asch, eds., pp. 259-270.

Fagan, K.A., Graf, R.A., Tolman, S., Schaack, J., and Cooper, D.M.F. (2000) Regulation of a Ca2+-Sensitive Adenylyl Cyclase in an Excitable Cell: Role of Voltage-Gated vs. Capacitative Ca2+ Entry. J. Biol. Chem. 275:40187-40194.

Smith, R.L., Traul, D.L., Schaack, J., Clayton, G.H., Staley, K.J., and Wilcox, C. (2000) Characterization of Promoter Function and Cell-Type Specific Expression from Viral Vectors in the Nervous System. J. Virol. 74:11254-11261.

Wan, Y.Y., Leon, R.P., Marks, R., Cham, C.M., Schaack, J., Gajewski, T.F., and DeGregori, J. (2000) Transgenic Expression of the Coxsackie/Adenovirus Receptor (CAR) Enables Adenoviral Mediated Gene Delivery in Naîve T cells. Proc. Natl. Acad. Sci. USA 97:13784-13789.

Thomas, D., Schaack, J., Vogel, H., and Javier, R. (2001) Several E4 Region Functions Influence Mammary Tumorigenesis by Human Adenovirus Type 9. J. Virol. 75:557-568.

Orlicky, D.J. and Schaack, J. (2001) Adenovirus Transduction of 3T3-L1 cells. J Lipid Res. 42:460-466.

Orlicky, D.J., DeGregori, J., and Schaack, J. (2001) Construction of Stable Coxsackievirus and Adenovirus Receptor Expressing 3T3-L1 Cells. J. Lipid Res. 42:910-915.

Fagan, K., Schaack, J., Zweifach, A., and Cooper, D.M.F. (2001) Adenovirus Encoded Cyclic Nucleotide-Gated Channels: A New Methodology for Monitoring cAMP in Living Cells. FEBS Lett., 500:85-90.

Rich, T.C., Tse, T.E., Rohan, J.G., Schaack, J., and Karpen, J.W. (2001) In vivo Assessment of Local Phosphodiesterase Activity using Tailored Cyclic Nucleotide-Gated Channels as cAMP Sensors. J Gen Physiol. 118:63-78.

Bennett, M. L., Galan, H., Owens, G., Dewey, A., Banks, R., Accurso, F., Hobbins, J., and Schaack, J. (2001) In Utero Gene Delivery by Intra-Amniotic Injection of a Retroviral Vector Producer Cell Line in a Non-Human Primate Model. Hu. Gene Ther., 12:1857-1865.

Rich, T.C., Fagan, K.A., Tse, T.E., Schaack, J., Cooper, D.M.F., and Karpen, J. W. (2001) A Uniform Stimulus Triggers Distinct cAMP Signals in Different Cellular Compartments. Proc. Natl. Acad. Sci. USA 98:13049-13504.

Klemm, D.J., Watson, P.A., Frid, M.G., Dempsey, E.C., Schaack, J., Colton, L.A., Nesterova, A., Stenmark, K.R., Reusch, J.E.-B. (2001) CREB Content is a Molecular Determinant of Smooth Muscle Cell Phenotype. J. Biol. Chem. 276:46132-46141.

Schaack, J., Allen, B., Orlicky, D., Bennett, M., Maxwell, I., and Smith, R.L. (2001) Promoter Strength in Adenovirus Transducing Vectors: Down-Regulation of the Adenovirus E1A Promoter in 293 Cells Facilitates Vector Construction. Virology 291:101-109.

Roman, R., Troetsch, M., Dunkelberg, J., Kilic, G., Schlenker, T., Schaack, J., and Fitz, J.G. (2002) Molecular characterization of volume-sensitive SKCa channels in human liver cells. Am. J. Physiol. Gastrointest. Liver Physiol. 282:G116-G122.

Allen, M.P., Linseman, D.A., Udo, H., Xu, M., Schaack, J., Varnum, B., Kandel, E.R., Heidenreich, K.A., and Wierman, M.E. (2002) A Novel Mechanism for GnRH Neuron Migration Involving Gas6/Ark Signaling to p38 MAP Kinase. Mol. Cell. Biol. 22:599-613.

Zhou, W, Schaack, J., Zawada, W., and Freed, C. (2002) Overexpression of human alpha-synuclein causes dopamine neuron death in primary human mesencephalic culture. Brain Research 926:42-50.

Cota-Gomez, A., Flores, N.C., Casullo, A., Cruz, C., Aw, T.Y., Ichikawa, H., Schaack, J., Scheinman, R., and Flores, S. C. (2002) The Human Immunodeficiency Virus-1 Tat Protein Activates Human Umbilical Vein Endothelial Cell E-selectin Expression via an NF-?B-Dependent Mechanism. J. Biol. Chem. 277:14390-14399.

Cioffi, D.L., Moore, T.M., Schaack, J., Creighton, J.R., Cooper, D.M., and Stevens, T. (2002) Dominant Regulation of Interendothelial Cell Gap Formation by Calcium-Inhibited Type 6 Adenylyl Cyclase. J. Cell. Biol. 157:1267-1278.

Galan, H.L., Bennett, M.L., Tyson, R. W., Owens, G., Regnault, T.R.H., Accurso, F., Hobbins, J., and Schaack, J. (2002) Inefficient Transduction of Sheep in utero following Intraamniotic Injection of Retroviral Packaging Cells. Am. J. Ob. Gyn. 187:469-474.

McManaman, J.L., Zabaronick, W., Schaack, J., and Orlicky, D. (2003) Identification of Lipid Droplet Targeting Domains of Adipose Differentiation Related Protein. J. Lipid Res. 278:625-635.

Russell, T.D., Fischer, A., Beeman, N.E., Freed, E.F., Neville, M.C., and Schaack, J. (2003) Transduction of the Mammary Epithelium with Adenoviral Vectors in vivo. J. Virol., 77:5801-5809.

Bennett, M.L. and Schaack, J. (2003) Development of a Dual-Luciferase Fusion Gene as a Sensitive Marker for Site-Directed DNA Repair Strategies. J. Gene Medicine 5:723-732.

Köhrer, C., Yoo,, J.-H., Bennett, M.L., Schaack, J., and RajBhandary, U.L. (2003) Concomitant suppression of amber and ochre codons in a single mRNA: A general approach to site-specific insertion of two different unnatural amino acids into proteins. Chem. and Biol., 10:1095-1102.

Schaack, J., Bennett, M.L., Colbert, J., Vazques Torres, A., Clayton, G.H., Ornelles, D., and Moorhead, J. (2004) E1A and E1B Proteins Inhibit Inflammation Induced by Adenovirus. Proc. Natl. Acad. Sci. USA, 101:3124-3129.

Qadri, I., Iwahashi, M., Capasso, J.M., Hopkins, M.W., Flores, S., Schaack, J., and Simon, F.R. (2004) Induced Oxidative Stress and Activated Expression of Manganese Superoxide Dismutase (MnSOD) during Hepatitis C Virus Replication: Role of JNK, p38MAPK &AP-1. Biochem. J., 378:919-928.

Schaack, J. (2005) Adenovirus Vectors Deleted for Genes Essential for Viral DNA Replication. Front. Biosci. 10:1146-1155.

Schaack, J. (2005) Induction and Inhibition of Innate Inflammatory Responses by Adenovirus Early Region Proteins. Viral Immunol. 18:79-88.

Qiao, L., MacLean, P.S., Schaack, J., Orlicky, D.J., Darimont, C., Pagliassotti, M., Friedman, J.E., and Shao, J. (2005) C/EBP? Regulates Human Adiponectiv Gene Transcription through an Intronic Enhancer. Diabetes 54:1744-1754.

Modiano, J.F., Lamerato-Kozicki, A.R.. Jubala, C.M., Coffey, D., Borakov, M., Schaack, J., and Bellgrau, D. (2005) Fas Ligand Gene Transfer for Cancer Therapy. Cancer Therapy, in press.

Ahmad, A., Ahmad, S., Chang, L.-Y., Schaack, J., and White, C.W. (2005) Endothelial Akt Activation by Hyperoxia: Role in Cell Survival. Free Radical Biology And Medicine, accepted.

Qiao, L., Schaack, J., and Shao, J Suppression of Adiponectin Gene Expression by Histone Deacetylase Inhibitor Valproic Acid. Endocrinology, accepted.

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