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Research Program The long-term goals of the research in this laboratory are to elucidate the causes of the initiation and progression of osteoarthritis and other degenerative joint diseases and to create and improve treatment and prevention strategies. The approach is multi-disciplinary using techniques from molecular biology, biochemistry, mechanobiology, cell biology, biomarker analysis, imaging, biomechanics, and tissue engineering.
The laboratory collaborates with the Musculoskeletal Disorders Lab in identifying inflammatory agents in soft tissue exposed to cyclic and static loading.
Facilities & Equipment Facilities Equipment Electrical muscle stimulator, A/D board, amplifier, data acquisition workstation, load cells HPLC workstation, software, auto-sampler, dual pumps, UV detector, fluorescence detector Vacuum centrifuge concentrator Multi-well plate reader with workstation and data analysis software, multi-well plate washer, multi-well plate shaker Histology tissue processor, paraffin embedder, rotary microtome, slide dryer Light and fluorescence microscope, digital camera, digital imaging and image analysis software Electrophoresis units, mini-electrophoresis units, electrical transfer units, semi-dry electrical transfer unit, power supply units Tissue dimembranator (tissue mill) Hybridization oven, UV cross-linker DNA/RNA horizontal gel electrophoresis units Cell culture incubators, bio-safety laminar flow hood Refrigerated centrifuge, micro-centrifuges Autoclave Electrical balances, pH meters -80 freezers, -20 freezer, refrigerator
Research Projects
Graduate Students
Research Residents Trevor Oren, MD, (2007-present) Arthroplasty outcomes in diabetics: bone metabolism and clinical biomarkers.
Medical Students Keely Chevalier (2008- ) Imran Choudhry (2007-2008) Jointly supervised with Dr. M. Solomonow. Julian Ku (2008- ) Jointly supervised with Dr. M. Solomonow.
Fellows Navid Ziran (2007-2008) Jointly supervised with Dr. D. Hak. The effect of age on in vivo bone formation by rat marrow stem cells.
Research Student Interns HoanVu Ngyuen (summer 2008) Rangeview High School Ananth Sridar (summer 2007) Stanford University
Publications Publications KB King, S Chubinskaya, DL Reid, LH Madsen, and J Mollenhauer. Absence of cell-surface annexin V is accompanied by defective collagen matrix binding in the Swarm rat chondrosarcoma. Journal of Cellular Biochemistry. 65:131-144, 1997.
J Mollenhauer, MT Mok, KB King, M Gupta, S Chubinskaya, H Koepp, and AA Cole. Expression of anchorin CII (cartilage annexin V) in human young, normal adult, and osteoarthritic cartilage. Journal of Histochemistry and Cytochemistry. 47:209-220, 1999.
L Jennings, L Wu, KB King, H Hämmerle, G Cs-Szabo, and J Mollenhauer. The effects of collagen fragments on the extracellular matrix metabolism of bovine and human chondrocytes. Connective Tissue Research. 42:71-86, 2001.
D Heinegård, K King, M Mörgelin, K Rosenberg, and C Wiberg. Matrix molecules with roles in cartilage assembly. In: Molecular Biology in Orthopaedics. RN Rosier and CH Evans, editors. American Academy of Orthopaedic Surgeons, Rosemont, IL, pp. 315-325, 2003.
KB King and JH Kimura. The establishment and characterization of an immortal cell line with a stable chondrocytic phenotype. Journal of Cellular Biochemistry. 89:992-1004, 2003.
KB King, CT Lindsey, MD Ries, LS Steinbach, and S Majumdar. A study of the relationship between molecular biomarkers of joint degeneration and the magnetic resonance-measured characteristics of cartilage in 16 symptomatic knees. Magnetic Resonance Imaging. 22:1117-1123, 2004.
A Mündermann, CO Derby, TP Andriacchi, and KB King. Serum concentration of cartilage oligomeric matrix protein (COMP) is sensitive to physiological cyclic loading in healthy adults. Osteoarthritis and Cartilage. 13:34-38, 2005.
LH Nakama, KB King, SO Abrahamsson, and D Rempel. Evidence of microtears due to cyclical loading in an in vivo tendinopathy model. Journal of Orthopaedic Research. 23:1199-1205, 2005.
KB King, C Opel, and D Rempel. Cyclical articular joint loading leads to cartilage thinning and osteopontin production in a novel in vivo rabbit model of repetitive finger flexion with loading. Osteoarthritis and Cartilage. 13:971-978, 2005.
LH Nakama, KB King, SO Abrahamsson, and D Rempel. VEGF, VEGFR-1, and CTGF cell densities in tendon are increased with cyclical loading: An in vivo tendinopathy model. Journal of Orthopaedic Research. 24:1-9, 2006.
C Li, LA Pruitt, and KB King. Nanoindentation differentiates tissue-scale functional properties of native articular cartilage. Journal of Biomaterials Research. 17A:729-738, 2006.
E Saadat, H Lan, S Majumdar, DM Rempel and KB King. Long-term cyclical in vivo loading increases cartilage proteoglycan content in a spatially specific manner: an infrared microspectroscopic imaging and polarized light microscopy study. Arthritis Research and Therapy. 8:R147, 2006.
LH Nakama, KB King, S Abrahamsson, DM Rempel. Effect of Repetition Rate on the Formation of Microtears in Tendon in an In Vivo Cyclical Loading Model. Journal of Orthopaedic Research. 25:1176-1184. 2007. KR Asundi, KB King, and DM Rempel. Evaluation of gene expression through qRT-PCR in cyclically loaded tendons: an in vivo animal model. European Journal of Applied Physiology. 102:265-270, 2008.
C Li, KB King, and LA Pruitt. Zone-specific changes in micromechanical, biochemical, and structural properties in articular cartilage from a rabbit joint flexion model. Materials Research Society Symposium Proceedings. 1097E:1-6, 2008.
Funding R01 OH007786 P30 DK57516
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