Tuesday, June 8, 2004 -- Evening
5:00-7:00 PM	Evening Registration -- Wine and Cheese Reception	Given Institute
Wednesday, June 9, 2004 -- Morning
8:00-8:15 AM	Welcome	Rubin M. Tuder, M.D., Chair Norbert F. Voelkel, M.D., Co-Chair Karen A. Fagan, M.D., Co-Chair
8:15-8:30 AM	Introduction	Thomas L. Petty, M.D.
MODERATOR - Norbert Voelkel, M.D.
8:30-9:15 AM	STATE OF THE ART "Phenotypic Characterization of Pulmonary Arteries in Normal and Diseased Lungs"	Professor Dr. Michael Kasper Institute fur Anatomie, Dresden, Germany
9:15-9:30 AM	Discussion
9:30-9:45 AM	SEROTONIN-INDUCED SMOOTH MUSCLE HYPERPLASIA IN VARIOUS FORMS OF HUMAN PULMONARY HYPERTENSION. E. Marcos*, E. Fadel§, O. Sanchez*, M. Humbert†, P. Dartevelle§, G. Simonneau†, M. Hamon#, S. Adnot*, S. Eddahibi*, Service de Physiologie Explorations Fonctionnelles, Hôpital H. Mondor, Créteil, FRANCE; #Neuro Psycho Pharmacologie Moléculaire, Cellulaire et Fonctionnelle, Faculté de Médecine Pitié-Salpêtrière, Paris, Cedex, FRANCE; §Service de Pneumologie, Hôpital A. Béclère, Clamart, FRANCE; †Service de Chirurgie Thoracique, Vasculaire et de Transplantation Cardiopulmonaire, Hôpital Marie Lannelongue, Le Plessis Robinson, FRANCE.
9:45-10:00 AM	SUPPRESSION OF BMPRII IN VASCULAR SMOOTH MUSCLE INDUCES PULMONARY ARTERIAL HYPERTENSION IN TRANSGENIC MICE. J. West*, K. Fagan, W. Steudel, Y. Tada, B. Fouty, J. Harral, M. Miller, J. Ozimek, R. Tuder, D. Rodman, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Health Sciences Center, Denver, CO.
10:00-10:30 AM	Coffee Break
MODERATOR – Ivan McMurtry, Ph.D.
10:30-11:15 AM	GILES F. FILLEY LECTURE "REAL-TIME, IN VIVO, VISUALIZATION OF PULMONARY MICROCIRCULATION" Jahar Bhattacharya, M.D., D.Phil. Director, Lung Biology Group Columbia University College of Physicians & Surgeons New York, New York
11:15-11:30 AM	Discussion
11:30-11:45 AM	SUPEROXIDE AND CHRONIC HYPOXIA-INDUCED PULMONARY HYPERTENSION IN NEW- BORN PIGLETS. C.D. Fike*, J.L. Aschner, Y. Zhang, D. Salvemini, M.R. Kaplowitz, Department of Pediatrics, Wake Forest University School of Medicine & Metaphore Pharmaceuticals, St. Louis, MO.
11:45-12:00	REDOX ACTIVATION OF INTRACELLULAR CALCIUM RELEASE CHANNELS (RYANODINE RECEPTORS) IN THE SUSTAINED PHASE OF HYPOXIA-INDUCED PULMONARY VASOCONSTRICTION. W. Du, M. Frazier, T.J. McMahon, J.P. Eu*, Division of Pulmonary, Allergy and Critical Care Medicine, Duke University Medical Center, Durham, NC.
12:00-1:30 PM	Lunch
Wednesday, June 9, 2004 -- Afternoon Moderator – Kurt Stenmark, M.D.
1:30-2:15 PM	STATE OF THE ART "Cellular and Molecular Heterogeneity Troy Stevens, Ph.D. of Pulmonary Endothelium" University of South Alabama
2:15-2:30 PM	Discussion
2:30-2:45 PM	THE EXTRACELLUAR MATRIX MICROENVIRONENT SPECIFIES PULMONARY ENDOTHELIAL CELL IDENTITY: ROLES OF TENASCIN-C & RHOA. L. Sisbarro1, K. Ihida-Stansbury1*, T. Stevens2, N. Bauer3, I. McMurtry3, P.L. Jones1, University of Colorado Health Sciences Center, 1Departments of Pediatrics and 3Medicine, Denver, CO; 2Center for Lung Biology, University of South Alabama, Mobile, AL.
2:45-3:00 PM	ABERRANT SIGNAL TRANSDUCTION IN PULMONARY HYPERTENSION. K.B. Lane*#, T.R. Blackwell#, J. Runo#, L. Wheeler#, J. Phillips^, J. Loyd#, #Division of Allergy, Pulmonary and Critical Care Medicine, ^Division of Medical Genetics, Vanderbilt University School of Medicine, Nashville, TN.
3:00-3:30 PM	Break
Moderator – Rubin Tuder, M.D.
3:30-4:15 PM	STATE OF THE ART "Cellular and Molecular Mechanisms in Carlyne D. Cool, M.D. Pulmonary Hypertension" University of Colorado Health Sciences Center
4:15-4:30 PM	Discussion
4:30-4:45 PM	DEFINITIVE EVIDENCE OF FUNDAMENTAL AND INHERENT ALTERATION IN THE PHENOTYPE OF PPH ENDOTHELIAL CELLS IN ANGIOGENESIS. F. Masri*, B.Anand-Apte‡, A. Vasanji§, W. Xu, T. Goggans*, J. Drazba§, S.C. Erzurum*, Departments of *Pulmonary and Critical Care, *Cancer Biology, ‡Department of Ophthalmic Research, Cole Eye Institute, §Imaging Core, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH.
4:45-5:00 PM	MAJOR CHANGES IN DIMETHYLARGININE REGULATION IN PULMONARY HYPERTENSION: INCREASED LEVELS OF ASYMMETRIC AND SYMMETRIC DIMETHYLARGININES AND REDUCED EXPRESSION OF DIMETHYLARGININE DIMETHYLAMINOHYDROLASE 2. R.T. Schermuly1*, S. Pullamsetti1, R. Voswinckel1, W. Klepetko2, C. Aigner2, O. Eickelberg1, H.A. Ghofrani1, L. Fink1, N. Weissmann1, F. Grimminger1, L. Kiss1, W. Seeger1, 1Department of Internal Medicine, Justus-Liebig-University Giessen, Giessen, GERMANY. 2Department of Cardiothoracic Surgery, University of Vienna, Vienna, AUSTRIA.
5:15-5:30 PM	THE PROTECTIVE ROLE OF T-LYMPHOCYTES IN PULMONARY VASCULAR REMODELING. L. Taraseviciene-Stewart*, D. Kraskauskas, R. Scerbavicius, N. Burns, M. Nicolls, N.F. Voelkel, Pulmonary Hypertension Center, Department of Pathology, University of Colorado Health Sciences Center, Denver, CO.
5:30 PM	POSTER VIEWING --- SOCIAL HOUR
Thursday, June 10, 2004 – Morning Moderator – Scott Worthen, M.D.
8:00-8:45 AM	THOMAS A. NEFF LECTURE "THE ROLE OF BONE MARROW ELEMENTS IN ANGIOGENSIS AND LUNG REPAIR" Shahin Rafii, M.D. Associate Professor of Medicine Department of Hematology and Oncology Cornell University Medical College New York, New York
8:45-9:00 AM	Discussion
9:00-9:15 AM	A SUBPOPULATION OF LEUKOCYTES WITH A DUAL, MACROPHAGE-FIBROBLAST-LIKE PHENOTYPE CONTRIBUTES TO HYPOXIA-INDUCED PULMONARY ARTERY ADVENTITIAL THICKENING. M.G. Frid*, J.A. Brunetti, D.L. Burke, T.C. Carpenter, N.J. Davie, K.R. Stenmark, University of Colorado Health Sciences Center, Denver, CO.
9:15-9:30 AM	MICROARRAY ANALYSIS OF PERIPHERAL BLOOD CELLS IN PULMONARY ARTERIAL HYPERTENSION, SURROGATE TO BIOPSY. T.M. Bull*, C.D. Coldren, S.M. Sotto-Santiago, M. Moore, N.F. Voelkel, M.W. Geraci, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Health Sciences Center, Denver, CO.
9:30-10:00 AM	Coffee Break
Moderator – Karen Fagan, M.D.
10:00-10:45 AM	STATE OF THE ART "The Molecular Basis of TGF-_ Control of Vascular Professor Dr. Peter ten Dijke Homeostasis and Vascular Diseases" Netherlands Cancer Institute, Amsterdam
10:45-11:00 AM	Discussion
11:00-11:15 AM	BMP4 PROMOTES VASCULAR REMODELING IN HYPOXIC PULMONARY HYPERTENSION. D. Frank, J. Johnson, M. de Caestecker*, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN.
11:15-11:30 AM	REGULATION AND FUNCTIONS OF THE PAIRED-RELATED HOMEOBOX GENE, Prx1, IN PULMONARY VASCULAR DEVELOPMENT AND DISEASE. K. Ihida-Stansbury1*, D.M. McKean1*, S.A. Gebb2, J.F. Martin3, T. Stevens4, R. Nemenoff2, J. Vaughn1, K. Lane5, J. Loyd5, L. Wheeler5, N.W. Morrell6, D. Ivy1, P.L. Jones1*, University of Colorado Health Sciences Center, Departments of 1Pediatrics and 2Medicine, Denver, CO; 3Texas A & M University, Institute of Biosciences and Technology, Houston, TX; 4Center for Lung Biology, University of South Alabama, Mobile AL; 5Vanderbilt University, Nashville, TN; 6Cambridge University, Department of Medicine, Cambridge,,UNITED KINGDOM.
11:30 AM	Instructions for Picnic
12:00 Noon	Picnic at East Maroon Bells Portal
Friday, June 11, 2004 – Morning Moderator – James Fisher, M.D.
8:00-8:45 AM	STATE OF THE ART "Regulation of Vascular Responses and Gregg L. Semenza, M.D., Ph.D. Vascular Homeostasis by Hypoxia" Johns Hopkins University School of Medicine
8:45-9:00 AM	Discussion
9:00-9:15 AM	IDENTIFICATION OF COMPLEX TEMPORAL PATTERNS OF GROWTH FACTORS, ADHESION MOLECULES AND ECM COMPONENTS IN A MOUSE MODEL OF HYPOXIC PULMONARY HYPERTENSION. S.A. Gharib1,2*, R.W. Glenny2, D.K. Madtes1, 1Fred Hutchinson Cancer Research Center, Seattle, WA and 2University of Washington, Seattle, WA.
9:15-9:30 AM	CHRONIC HYPOXIA INDUCED MURINE PULMONARY HYPERTENSION: ROLE OF SUPEROXIDE AND gp91phox. J.Q. Liu*, R.J. Folz, Division of Pulmonary, Allergy, and Critical Care Medicine, Departments of Medicine and Cell Biology, Duke University Medical Center, Durham, NC.
9:30-10:00 AM	Coffee Break
Moderator – York Miller, M.D.
10:00-10:45 AM	STATE OF THE ART "Use of Phage Display to Define Renata Pasqualini, Ph.D. Endothelial Cell Phenotypes" University of Texas MD Anderson Cancer Center
10:45-11:00 AM	Discussion
11:00-11:15 AM	HYPOXIA AMPLIFIES THE PROLIFERATIVE CAPACITY OF DISTAL HUMAN PULMONARY ARTERY SMOOTH MUSCLE CELLS. E.J. Growcott1*, K.H. Banner2, J. Wharton1, 1Imperial College London, Hammersmith Campus, London, UNITED KINGDOM and 2Pfizer Global Research & Development, Sandwich, Kent, UNITED KINGDOM.
11:15-11:30 AM	ABNORMAL VASCULAR PHENOTYPES IN PATIENTS WITH IPF AND SECONDARY PULMONARY HYPERTENSION. J. Gagermeier*, J. Dauber, S. Yousem, K. Gibson, N. Kaminski, Simmons Center for Interstitial Lung Disease; University of Pittsburgh Medical Center, Pittsburgh, PA.
11:30-11:45 AM	DOES BMPR2 MUTATION DISRUPT PULMONARY VASCULOGENESIS? T.K. Jeffery, P.D. Upton, X. Yang, M. Southwood, L. Long, R.C. Trembath, N.W. Morrell*, Department of Medicine, University of Cambridge School of Clinical Medicine, Addenbrooke’s and Papworth Hospitals, Cambridge, UNITED KINGDOM.
11:45-1:30 PM	Lunch
Friday, June 11, 2004 – Afternoon Moderator – Mark Geraci, M.D.
1:30-2:15 PM	PARKER B. FRANCIS LECTURESHIP "IMAGING OF ANGIOGENIC PROCESSES" Donald M. McDonald, M.D., Ph.D. Professor, Department of Anatomy Cardiovascular Research Institute and Comprehensive Cancer Center University of California, San Francisco Medical Center San Francisco, California
2:15-2:30 PM	Discussion
2:30-2:45 PM	EXTRACELLULAR ATP: A POTENTIAL REGULATOR OF VASA VASORUM NEO- VASCULARIZATION IN HYPOXIA-INDUCED PULMONARY VASCULAR REMODELING. E. Gerasimovskaya1*, N. Davie1, S. Ahmad2, D. Tucker1, C. White2, K.R. Stenmark1, 1University of Colorado Health Sciences Center, Denver, CO.
2:45-3:00 PM	Rho GTPases IN LUNG DEVELOPMENT AND PULMONARY HYPERTENSION. J. Kaufman, E. Sakao, M. Oka, T. Nagaoka, C. Oliver-Pickett, P.L. Jones, I.F. McMurtry, S.A. Gebb*, Departments of Medicine and Pediatrics, University of Colorado Health Sciences Center, Denver, CO.
3:00-3:30 PM	Break
Moderator – Edward Dempsey, M.D.
3:30-4:15 PM	STATE OF THE ART "Role of Apoptosis in the Pulmonary Circulation" Peter M. Henson, Ph.D. National Jewish Medical and Research Center
4:15-4:30 PM	Discussion
4:30-4:45 PM	VEGF RECEPTOR BLOCKADE BY SU5416 COMBINED WITH PULSATILE SHEAR STRESS CAUSES APOPTOSIS AND SUBSEQUENT PROLIFERATION OF APOPTOSIS-RESISTANT ENDOTHELIAL CELLS. S. Sakao*, L. Taraseviciene-Stewart*, C.D. Cool#, N.F. Voelkel*, The Pulmonary Hypertension Center* and Department of Pathology#, University of Colorado Health Sciences Center, Denver, CO.
4:45-5:00 PM	GENOMIC THREATS FROM PHYSIOLOGICAL SIGNALS IN LUNG VASCULAR CELLS. M.N. Gillespie, K.A. Ziel, V. Grishko, C.C. Campbell, G.L.Wilson*, University of South Alabama, College of Medicine, Departments of Pharmacology and of *Cell Biology and Neuroscience, and the Center for Lung Biology, Mobile, AL.
5:00-5:15 PM	A POTENTIAL ROLE FOR VARIABLE NUMBER TANDEM REPEATS IN THE PROSTACYCLIN SYNTHASE PROMOTER IN PRIMARY PULMONARY HYPERTENSION. S.P. Nana-Sinkam1*, R.J. Oyer1, R.S. Stearman1, S. Sotto-Santiago1, M.D. Moore1, K. Lane2, J.E. Loyd2, M.W. Geraci1, 1Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Health Sciences Center, Denver, CO; 2Division of Allergy, Pulmonary and Critical Care Medicine, Vanderbilt University Medical Center, Nashville, TN.
5:15-7:15 PM	POSTER VIEWING -- WINE AND CHEESE RECEPTION
Saturday June 12, 2004 – Morning Moderator – Marvin Schwarz, M.D.
8:00-8:45 AM	ROGER S. MITCHELL LECTURE "CLINICAL CHALLENGES IN PULMONARY HYPERTENSION" Gérald Simonneau, M.D. Division of Pulmonary and Critical Care Medicine Antoine Beclere Hospital, Clamart Paris-Sud University Clamart, France
8:45-9:00 AM	Discussion
9:00-9:15 AM	INTERVENTRICULAR MECHANICAL ASYNCHRONY DUE TO RIGHT VENTRICULAR PRESSURE OVERLOAD IN PULMONARY HYPERTENSION PLAYS AN IMPORTANT ROLE IN IMPAIRED LEFT VENTRICULAR FILLING. A. Vonk Noordegraaf*, J.T. Marcus, T. Gan, A. Boonstra, P.E. Postmus, VU Medical Center, Department of Pulmonary Medicine, Amsterdam, THE NETHERLANDS.
9:15-9:30 AM	MAGNETIC RESONANCE IMAGING PULMONARY ANGIOGRAMS. M.R. Pritzker*, J. Lesser, T. Long, Minneapolis Heart Institute Foundation, Minneapolis, MN.
9:30-9:50 AM	ENHANCED HYPOXIC PULMONARY VASOCONSTRICTION IN FAMILIES OF ADULTS OR CHILDREN WITH PRIMARY PULMONARY HYPERTENSION. E. Grünig1*, CH Dehnert2, R. Zimmerman3, R. Koehler4, M. Gorenflo5, R. Naeije6, H. Olschewski7, W. Nicols8, H.A. Katus1, D. Schranz3, B. Janssen3, Department of Cardiology1, Institute of Sports Medicine2, Department of Pediatric Cardiology5, Institute of Human Genetics4, University of Heidelberg, Heidelberg, GERMANY; Department of Pediatric Cardiology3 and Pneumology7, University of Giessen, Giessen; Division of Human Genetics8, Children’s Hospital, Cincinnati, Ohio, USA; Department of Pneumology, University of Bruxelles, BELGIUM6. 9:50-10:15 AM Coffee Break
Moderator – David Riches, Ph.D.
10:15-11:00 AM	STATE OF THE ART "Role of Growth Factors in Pulmonary Hypertension" Duncan J. Stewart, M.D. University of Toronto, Ontario, Canada
11:00-11:15 AM	Discussion
11:15-11:30 AM	ELEVATED LEVELS OF MATRIX METALLOPROTEINASES (MMP) IN THE URINE OF PATIENTS WITH PULMONARY ARTERIAL HYPERTENSION. J.I. Benisty*, J. Folkman*, D. Zurakowski*, S. Kilroy*, G. Louis*, D. Langleben§, S. Rich‡, M.A. Moses*, Vascular Biology Program, The Children’s Hospital (Boston), Harvard Medical School*, and SMBD-Jewish General Hospital§, Montreal, CANADA, and Rush Heart Institute, Center for Pulmonary Heart Disease‡, Chicago, IL.
11:30-12:30 PM	CONFERENCE SUMMARY Marlene Rabinovitch, M.D. Dwight and Vera Dunlevie Professor of Pediatrics Research Director of the Wall Center for Pulmonary Hypertension Stanford University School of Medicine Stanford, California

POSTERS

THE ROLE OF p38 MAP KINASE HYPOXIA-INDUCED VASCULAR CELL PROLIFERATION: AN INTERSPECIES COMPARISON. D. Welsh*, H. Mortimer, A. Kirk1, A. Peacock, Scottish Pulmonary Vascular Unit, Department of Surgery1, Western Infirmary, Glasgow, UNITED KINGDOM.

IL-6 CAUSES MILD PULMONARY HYPERTENSION AND AUGMENTS HYPOXIA-INDUCED PULMONARY HYPERTENSION IN MICE. S. Golembeski, J. West, Y. Tada, K.A. Fagan*, University of Colorado Health Sciences Center, Center for Genetic Lung Diseases, Cardiovascular Pulmonary Research Laboratory, Denver, CO.

EFFECTS OF SIMVASTATIN ON CIGARETTE SMOKING-INDUCED STRUCTURAL AND FUNCTIONAL CHANGES IN RAT LUNGS. S.D. Lee1*, J.H. Lee2, E.K. Kim2, K.H. Choi3, Y.M. Oh1, T.S. Shim1, 1Division of Pulmonary and Critical Care Medicine, Asan Medical Center, University of Ulsan, Seoul, KOREA; 2Department of Internal Medicine, Bundang CHA Hospital, Seoul, KOREA; 3Department of Internal Medicine, College of Medicine, Chungbuk National University, Cheongju, KOREA.

THE EXPRESSION OF PROSTACYCLIN SYNTHASE IS DECREASED IN THE SMALL PULMONARY ARTERIES FROM PATIENTS WITH EMPHYSEMA. J.D. Lee*, L. Taraseviciene-Stewart, R. Keith, M.W. Geraci, N.F. Voelkel, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Health Sciences Center, Denver, CO.

PULMONARY CAPILLARY HEMANGIOMATOSIS: RESULTS OF GENE EXPRESSION ANALYSIS. S.M. Kawut*, A.M. Assaad, S.M. Arcasoy, E.B. Rosenzweig, J.R. Sonett, A.C. Borczuk, College of Physicians and Surgeons, Columbia University, New York, NY.

PULMONARY CAPILLARY HEMANGIOMATOSIS: AN IMMUNOHISTOCHEMICAL ANALYSIS OF VASCULAR REMODELING IN A FATAL CASE. A. Sullivan4*, K. Chmura1, C.D. Cool4,6, N. Voelkel4, E.D. Chan1,2,3,4, 1Department of Medicine, 2Program in Cell Biology, National Jewish Medical and Research Center, 3Denver Veterans Administration Medical Center, 4Division of Pulmonary Sciences and Critical Care Medicine, 5Division of Cardiology, and 6Department of Pathology, University of Colorado School of Medicine, Denver, CO.

PROTEIN KINASE C INHIBITS cAMP-INDUCED BKCa CHANNEL ACTIVITY IN FAWN-HOODED RAT PULMONARY ARTERIAL SMOOTH MUSCLE VIA PHOSPHODIESTERASES. S.A. Barman*, S. Zhu, M.L. Meadows, R.E. White, Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta, GA

MTS1/S100A4 STIMUILATES HUMAN PULMONARY ARTERY SMOOTH MUSCLE CELL (HPASMC) MIGRATION THROUGH MULTIPLE SIGNALING PATHWAYS. E. Spiekerkoetter1*, A. Lawrie1, S. Merklinger1, N. Ambartsumian2, E. Lukanidin2, A-M. Schmidt3, M. Rabinovitch1, 1Department of Pediatrics, Stanford University School of Medicine; 2Department of Molecular Cancer Biology, DENMARK; 3Department of Physiology, Medicine and Surgery, Columbia University, New York, NY.

A PDGF-SELECTIVE TYROSINE KINASE INHIBITOR DIMINISHES THE DEVELOPMENT OF PULMONARY ARTERY HYPERTENSION IN RATS. J.A. Lasky*, S. Thammasitboon, Y. Zhuo, F. Jamous, J. Spees, S.R. Baber, M.A. Laboy, P.J. Kadowitz, Tulane University Health Sciences Center, New Orleans, LA.

GENERATION OF REACTIVE OXYGEN SPECIES (ROS) IN FETAL PULMONARY ARTERY (PA) AND VEIN (PV) SMOOTH MUSCLE (SMC) DURING HYPOXIA. F. Sander, Y. Gao, J.U. Raj*, Harbor-UCLA Research and Education Institute, Torrance, CA.

MECHANISMS OF HO-1-MEDIATED CARDIAC PROTECTION IN CHRONIC HYPOXIA: ROLE OF CARBON MONOXIDE AND BILIRUBIN. S.H. Vitali*, S.A. Mitsialis, H. Christou,, M. Kluger, A. Fernandez-Gonzalez, X. Liu, S. Kourembanas, Children’s Hospital Boston, Boston, MA.

DIFFERENTIAL GENE EXPRESSION IN CHRONIC HYPOXIC PULMONARY HYPERTENSION: EFFECT OF SIMVASTATIN TREATMENT. R.E. Girgis*, S.F. Ma, S.Q. Ye, D.N. Grigoryev, D. Li, P.M. Hassoun, R.M. Tuder, R.A. Johns, J.G.N. Garcia, Johns Hopkins University, Baltimore, MD.

ATTENUATION OF HYPOXIA-INDUCED PULMONARY ARTERY REMODELING BY A PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR-_ AGONIST. J.T. Crossno, Jr.*, K.G. Morris, Jr., D.J. Klemm, Division of Pulmonary Sciences and Critical Care Medicine, Cardiovascular Pulmonary Laboratory, University of Colorado Health Sciences Center and Denver VAMC, Denver, CO.

ENDOTHELIN-B RECEPTOR OVEREXPRESSION PREVENTS HYPOXIC PULMONARY HYPERTENSION IN THE CIRRHOTIC RATS. M. Imamura*, A.M. Vitello, J.N. Limbird, D.D. Ivy, M.B. Fallon, E.P. Carter, CVP Research Laboratory, University of Colorado Health Sciences Center, Denver, CO and University of Alabama, Birmingham, AL.

DIRECT NON-ENZYMATIC INTERACTION BETWEEN NEPRILYSIN, A CELL-SURFACE PEPTIDASE, AND INTRACELLUAR PROTEINS: A NOVEL SIGNALING MECHANISM FOR REGULATION OF PULMONARY ARTERY SMOOTH MUSCLE CELL PHENOTYPE. M.J. Wick*, Y.E. Miller, E.C. Dempsey, CVP Research Laboratory, University of Colorado Health Sciences Center and Denver VA Medical Center, Denver, CO.

THE LOW-VOLTAGE-ACTIVATED CALCIUM CHANNEL CA_3.1 CONTROLS PROLIFERATION OF HUMAN PULMONARY ARTERY MYOCYTES. D.M. Rodman, J. Harral, B.W. Fouty, S. Wu§, J. West, M. Hoedt-Miller, Y. Tada, K.A. Reese*, C. Cool¥, K.A. Fagan, L. Cribbs†, Center for Genetic Lung Disease, Division of Pulmonary Sciences and Critical Care Medicine, and ¥Department of Pathology, University of Colorado Health Sciences Center, Denver, CO, §University of South Alabama, Mobile, AL, †Loyola University, Chicago, IL.

HYPOXIA-INDUCED ALTERATIONS IN PKC_ SIGNALING RESULT IN AUGMENTED FIBROBLAST PROLIFERATION. M. Short, S. Fox, K.R. Stenmark, M. Das*, Department of Pediatrics, University of Colorado Health Sciences Center, Denver, CO.

ROLE OF RHO IN INCREASED MIGRATION OF PULMONARY ARTERY SMOOTH MUSCLE CELLS IN THE NEPRILYSIN NULL MOUSE, A MURINE MODEL OF EXAGGERATED HYPOXIC PUMONARY HYPERTENSION. V. Karoor*, S. Walchak, Y. Miller, E.C. Dempsey, CVP Research Laboratory, University of Colorado Health Sciences Center and Denver VA Medical Center, Denver, CO.

PULMONARY ENDOTHELIN-1 CLEARANCE IN HUMAN PULMONARY ARTERIAL HYPERTENSION. D. Langleben*, J. Dupuis, A. Hirsch, M. Giovinazzo, I. Langleben, J. Khoury, N. Ruel, A. Caron, Jewish General Hospital and Montreal Heart Institute, Montreal, CANADA.

POSTERS

TISSUE FACTOR IS INDUCED IN A RODENT MODEL OF SEVERE PULMONARY HYPERTENSION CHARACTERIZED BY NEOINTIMAL LESIONS TYPICAL OF HUMAN DISEASE. R.J. White*, I.I. Galaria, J. Harvey, B.C. Blaxall, C.D. Cool, M.B. Taubman, Pulmonary and Critical Care Medicine, Center for Cellular and Molecular Cardiology, Aab Institute for Biomedical Sciences, University of Rochester School of Medicine; Department of Pathology, University of Colorado Health Sciences Center, Denver, CO.

INHALED NITRIC OXIDE REVERSES HYPOXIA INDUCED LUNG HYPOPLASIA IN eNOS DEFICIENT MICE. V. Balasubramaniam*, A. Maxey, S. Abman, Pediatric Heart Lung Center, Department of Pediatrics, University of Colorado Health Sciences Center, Denver, CO

VASCULAR ENDOTHELIAL GROWTH FACTOR IMPROVES PULMONARY VASCULAR REACTIVITY AND STRUCTURE IN AN EXPERIMENTAL MODEL OF CHRONIC PULMONARY HYPERTENSION IN FETAL SHEEP. T.R. Grover*, T.A. Parker, S.H. Abman, Pediatric Heart Lung Center and Department of Pediatrics, University of Colorado School of Medicine, Denver, CO.

ROBUST EXPRESSION OF ANGIOPOIETIN IN NORMAL HUMAN LUNG WITH DIFFERENTIAL CHANGES IN PULMONARY ARTERIAL HYPERTENSION (PAH). A.E. Dutly+, L. Kugathasan, G. Proteau, M. Robb, T.K. Waddell+, S. Keshavjee+, D.J. Stewart*, Terrence Donnelly Research Laboratory, St. Michael’s Hospital and the Division of Thoracic Surgery+, Toronto General Hospital, University of Toronto, Ontario, CANADA.

PROTECTION AGAINST HYPOXIA-INDUCED PULMONARY ARTERIAL HYPERTENSION (PAH) THROUGH ANGIOPOIETIN-1 GENE TRANSFER. L. Kugathasan*, Y. Zhao, Y. Deng, D.J. Stewart, The Terrence Donnelly Heart Center, St. Michael’s Hospital, Toronto, Ontario, CANADA.

COMPARATIVE ANALYSIS OF BMPR2 GENE AND ITS MUTATIONS IN IDIOPATHIC PULMONARY ARTERIAL HYPERTENSION. W.P.K Wong1, J.A. Knowles2,3*, J.H. Morse1, 1Department of Medicine, 2Department of Psychiatry, Columbia University College of Physicians and Surgeons, New York, NY; 3New York State Psychiatric Institute, New York, NY.

VENTILATION-PERFUSION RATIOS IN PULMONARY ARTERIAL HYPERTENSION. EFFECTS OF INTRAVENOUS AND INHALED PROSTACYCLIN DERIVATIVES. T. Bratel1*, L. Lagerstrand2, L-A. Brodin2, J. Nowak2, I. Randmaa2, 1Department of Pulmonary Diseases, Karolinska Hospital; 2Department of Clinical Physiology, Huddinge University Hospital, Stockholm, SWEDEN.

EFFECTS OF AEROSOL VS. INTRAVENOUS UT15 ON PGH2-ANALOG INDUCED PULMONARY HYPERTENSION IN SHEEP. B.L. Sandifer*, K.L. Brigham, R.E. Parker, Center for Translational Research in the Lung, Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Emory University, Atlanta, GA.

THE IMPACT OF PULMONARY HYPERTENSION ON SURVIVAL IN PATIENTS WITH IDIOPATHIC PULMONARY FIBROSIS. H.F. Nadrous*, P.A. Pellikka, M.J. Krowka, K.L. Swanson, N. Chaowalit, P.A. Decker, J.H. Ryu, Mayo Clinic, Rochester, MN.

CHARACTERIZATION OF ACUTE HEMODYNAMIC EFFECTS OF THREE DIFFERENT PHOSPHODIESTERASE-5 INHIBITORS IN PATIENTS WITH PULMONARY ARTERIAL HYPERTENSION: A RANDOMIZED PROSPECTIVE STUDY. H.A. Ghofrani*, R. Voswinckel, F. Reichenberger, M.G. Kohstall, H. Olschewski, R.T. Schermuly, N. Weissmann, W. Seeger, F. Grimminger, Department of Internal Medicine, Justus-Liebig-University, Giessen, Giessen, GERMANY.

EVIDENCE FOR SYSTEMIC ENDOTHELIAL DYSFUNCTION IN PATIENTS AND FIRST ORDER RELATIVES WITH PULMONARY ARTERIAL HYPERTENSION. R. Hughes, J. Tong, C. Oates, J. Lordan, P.A. Corris*, Regional Cardiothoracic Centre & Regional Medical Physics Department, Freeman Hospital, Newcastle-upon-Tyne, England, UK.

BMPR2 MUTATIONS IN ADULTS AND CHILDREN WITH IDIOPATHIC PULMONARY ARTERIAL HYPERTENSION: ASSOCIATION WITH THYROID DISEASE. K.E. Roberts1, R.J. Barst2, J.J. McElroy3, K. Chada5, J.A. Knowles3,4*, J.H. Morse1, 1Departments of Medicine, 2Pediatrics and 3Psychiatry, Columbia University College of Physicians and Surgeons and the 4New York State Psychiatric Institute, New York, NY and the 5Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ.

ACUTE CARDIOPULMONARY HEMODYNAMIC EFFECTS OF BRAIN NATRIURETIC PEPTIDE IN PATIENTS WITH PULMONARY ARTERIAL HYPERTENSION. J.R. Klinger*, J. Houtchens, Sejal Thaker, N.S. Hill. H. Farber, Division of Pulmonary Medicine, Rhode Island Hospital, Providence, RI, New England Medical Center and Boston University Medical Center, Boston, MA.

SEROTONIN TRANSPORTER GENE POLYMORPHISM IN A COHORT OF GERMAN PATIENTS WITH IDIOPATHIC PULMONARY ARTERIAL (IPAH) OR CHRONIC THROMBOEMBOLIC PULMONARY HYPERTENSION (CTEPH). R. Koehler1, H. Olschewski4, M.M. Hoeper3, E. Grünig2, B. Janssen1*, 1Institute of Human Genetics, 2Department of Cardiology, University of Heidelberg, Heidelberg, GERMANY, 3Department of Pneumology, University of Hannover, GERMANY, 4Department of Pneumology, University of Giessen, Giessen, GERMANY.

METASTATIC CANCER WHILE ON CONTINUOUS PROSTACYCLIN THERAPY. K.A. Fagan*, T.M. Bull, D.B. Badesch, N.F. Voelkel, University of Colorado Health Sciences Center, Pulmonary Hypertension Center, Denver, CO.

DIVERGENT CONTRACTILE AND STRUCTURAL RESPONSES OF THE MURINE PKC-EPSILON NULL PULMONARY CIRCULATION TO CHRONIC HYPOXIA. C.M. Littler*, C.A. Wehling, K.A. Fagan, R.O. Messing, E.C. Dempsey, CVP Research Laboratory, University of Colorado Health Sciences Center and Denver VA Medical Center, Denver, CO and Ernest Gallo Clinic and Research Center, Department of Neurology, University of California San Francisco, Emeryville, CA.

OSTEOPONTIN MAY BE RESPONSIBLE FOR PULMONARY VASCULAR REMODELING. Y. Matsuda1, Y. Hoshikawa1*, S. Suzuki1, Y. Okada1, T. Tabata1, T. Sugawara1, M.W. Geraci2, N.F.Voelkel2, T. Kondo1, 1Department of Thoracic Surgery, IDAC, Tohoku University, Sendai, JAPAN; 2Division of Pulmonary and Critical Care Medicine, University of Colorado Health Sciences Center, Denver, CO.

EVIDENCE FOR VASCULAR REMODELING IN THE LUNGS OF MACAQUES INFECTED WITH SIV/HIV NEF RECOMBINANT VIRUS. J. Marecki*, C. Cool, N. Voelkel, P. Luciw, S. Flores, CVP Research Laboratory, and Pulmonary Hypertensive Center, University of Colorado Health Sciences Center, Denver, CO; California Primate Research Center, University of California, Davis, Davis, CA; Webb-Waring Institute, Denver, CO.