Jim Haughian Education: BS Dairy Science, 2000 MS Endocrinology/Physiology, 2002 University of Wisconsin at Madison E-mail: James.Haughian@uchsc.edu Lab Phone: 303-724-3513 Thesis Advisor: Andrew Bradford

Research Interests:

The Bradford lab is interested in cancer of the uterus, also known as endometrial cancer. Like other cancers, aberrant cell survival and apoptosis underlie the formation of uterine tumors. Our approach to understanding the formation of endometrial tumors has been to determine how the Protein Kinase C (PKC) family of signaling enzymes functions to determine survival and apoptosis in endometrial cancer cells. In endometrial cancer cell lines, we have observed that two different PKC isoforms, PKCd and PKCa, have opposing functional roles. In response to an apoptotic insult like DNA damage, our data indicate that PKCd is activated to mediate pro-apoptotic signaling. Under these same conditions, however, blocking PKCa enhances apoptosis, suggesting PKCa mediates survival signaling. We are currently working to determine a mechanism for how these PKC isozymes elicit their differential effects, as well as to determine whether the level of expression and activity of these PKC isozymes impacts tumor development.

General Interests:

My time outside the lab is dedicated to maintaining plant life on my plot of parched earth on the High Plains of Colorado. I also enjoy spending time with the numerous animals maintained on this same plot of land.

Publications:

Haughian, JM, Jackson, TA, Koterwas, DM and Bradford AP. Endometrial Cancer Cell Survival and Apoptosis is Regulated by Protein Kinase C Alpha and Delta. Endocrine-Related Cancer 2006, in press.

Sartori R, Haughian JM, Shaver RD, Rosa GJ, Wiltbank MC. Comparison of ovarian function and circulating steroids in estrous cycles of Holstein heifers and lactating cows. J Dairy Sci. 2004 Apr;87(4):905-20.

Haughian JM, Ginther OJ, Kot K, Wiltbank MC. Relationships between FSH patterns and follicular dynamics and the temporal associations among hormones in natural and GnRH-induced gonadotropin surges in heifers. Reproduction. 2004 Jan;127(1):23-33.

Navratil, A.M., S.P. Bliss, K.A. Berghorn, J.M. Haughian, T.A. Farmerie, J.K. Graham, C.M. Clay, M.S. Roberson. Constitutive localization of the gonadotropin-releasing hormone (GnRH) receptor to low-density membrane microdomains is necessary for GnRH signaling to ERK. J Biol Chem. 2003 Aug 22;278(34):31593-602. Epub 2003 Jun 05.

Haughian, J.M., R. Sartori, A. Gumen, J. N. Guenther, M. C. Wiltbank. Extending the postpartum anovulatory period in lactating dairy cattle with estradiol cypionate. J Dairy Sci 2002; 85:3238-3249.

Haughian, J. M. and M. C. Wiltbank. GnRH: From physiology to 'synch'-ology. Society for Theriogenology; 2002 Conference Proceedings, Colorado Springs, CO. 221-235.