Fig. 2. Expression of Sonic hedgehog, Patched and Gli1 in intermediate and late stage developing tongues. (A-C) Dorsal views of E14, C-R length 10.5-11.2 mm, and E14.5, C-R length 11-12 mm, tongues assayed for Shh, Ptc and Gli1 expression by whole mount in situ hybridization. (A) E14.5 tongue showing Shh expression predominantly in developing circumvallate (arrow) and fungiform (arrowheads) papillae. (B) E14.5 tongue, Ptc expression. (C) E14 tongue, Gli1 expression. Both Ptc and Gli1 expression mirror Shh expression. (D-G) Dorsal views of E16.5, C-R length 15-16.5 mm, tongues assayed for Shh and Ptc expression by whole mount in situ hybridization. (D) E16.5 tongue showing Shh expression predominantly in circumvallate (arrow) and fungiform (arrowheads) papillae. (E) Detail of fungiform papillae showing Shh expression. (F) E16.5 tongue showing Ptc expression. (G) Detail of fungiform papillae showing Ptc expression. Note the larger area of Ptc expression compared to Shh and the lower Ptc expression in the central core of the papilla. Scale bars: (A-D, F) 500 µm; (E,G) 50 µm.
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