Fig. 3. Shh and Ptc are expressed in different tissue types in early and intermediate stage tongues. Sagittal sections of E12.5 (A, B) and E14 (C-F) tongues assayed for Shh (A, C, E) or Ptc (B, D, F) expression by in situ hybridization. (A) Shh is expressed throughout the lingual epithelium at E12.5. (B) Ptc is expressed in both the lingual epithelium and mesenchyme. (C) E14 tongue showing Shh expression in a developing fungiform papilla. (D) At E14, Ptc is expressed in the fungiform epithelium and in underlying mesenchyme. (E) Shh expression in E14 circumvallate papillary epithelium. (F) Ptc is expressed in E14 circumvallate epithelium and mesenchyme. Scale bars: 50 µm.
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