Fig. 4. Shh and Ptc are expressed in taste papillae throughout gestation. Sagittal sections of E15-E18 tongues assayed for Shh and Ptc expression by in situ hybridization. (A) E16 tongue showing Shh expression in the central core of a fungiform papilla. (B) E16.5 tongue showing a broader region of Ptc expression surrounding a fungiform papilla. (C) E15 tongue showing Shh expression in the circumvallate epithelium. (D) E 16.5 tongue showing both epithelial and mesenchymal Ptc expression around the circumvallate papilla. (E) Shh expression at the surface of an E18 fungiform papilla. (F) Ptc is also expressed in E18 fungiform papillae. Scale bars: 50 µm.
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