FLORES, Sonia C., PhD         Pulmonary Science & Critical Care Medicine      Webb-Waring Institute 1W19      303-315-0055      sonia.flores@uchsc.edu

• HIV-1 Tat transcriptional protein and its pro-inflammatory effects on endothelial cells
• Oxidant/antioxidant balance in HIV infection
• Molecular mechanisms of human Mn-superoxide dismutase repression by HIV-1 Tat protein
• Redox modulation of transcriptional factor activity

• Promoter-reporter expression assays
• Electrophoretic mobility shift assays
• DNase footprinting assays
• Adenovirus transductions
• Tissue culture
• Tat-transgenic mouse technology to assess pulmonary complications in AIDS

• Komatsu S, Flores SC, Gerritsen ME, Anderson DC, and Granger DN. Differential up-regulation of cirucluating soluble and membrane-bound forms of murine intercellular adhesion molecule-1. American Journal of Pathology 151:205-14, 1997.
• Ichikawa H, Flores SC, Kvietys PR, Wolf RE, Yoshikawa T, Granger DN, and Aw TK. Molecular mechanisms of anoxia/reoxygenation-induced neutrophil adherence to cultured endothelial cells. Circulation Research, 81:922-931, 1997.

• Horie Y, Wolf R, Flores SC, McCord JM, Epstein CJ and Granger DN. Transgenic mice with increased copper/zinc-superoxide dismutase activity are resistant to hepatic leukostasis and capillary no-reflow after gut ischemia/reperfusion. Circ. Res. 83:691-696, 1998.

• Seve M, Favier A, Osman M, McCord JM, and Flores SC. The Tat protein from HIV-1 increases sensitivity to TPEN-induced apoptosis and changes Sp1 binding to DNA in HeLa cells. Archives of Biochemistry and Biophysics, 361:165-172, 1999.

• Vaitaitis GM, Sanderson RJ, Kimble EJ, Elkins ND and Flores SC. Modification of enzyme-conjugated streptavidin-biotin Western blot technique to avoid detection of endogenous biotin-containing proteins. Biotechniques 26 (5):854-8, 1999.

• Faust-Chan R, Hybertson B, Flores SC, Wright RM, Repine JE Initiation and tolerance to acute lung injury: yin-yang mechanisms involving interleukin-1. Chest 116 (1 Suppl): 102S-103S 1999.

• Oshima T, Flores SC, Vaitaitis G, Coe L, Joh T, Park J H, Zhu Y, Alexander B, Alexander, JS. Human immunodeficiency virus-1 Tat protein increases endothelial solute permeability through tyrosine kinase and MAP kinase-dependent pathways. AIDS 14: 1-8, 2000.

• Richard MJ, Guiraud P, Didier C, Seve M, Flores SC and Favier A. Human immunodeficiency virus-1 Tat protein impairs selenoglutathione peroxidase expression and activity by a mechanism independent of cellular selenium uptake: consequences on cellular resistance to UV-A radiation. Arch. Biochem. Biophys. 386:213-220, 2001.

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