RESEARCH INTERESTS:

• Acute lung injury (ARDS) and Chronic lung injury (COPD)
• Xanthine oxidase
• Neutrophil bactericidal/cytotoxic function
• Toxic oxygen metabolites
• Antioxidants
• O2 metabolite/elastase/antiprotease interactions
• Cytokines
• Ischemia/reperfusion
• Age-Related Macular Degeneration
• Cigarette smoking
• Mitochondria aging

• Isolated perfused lung/heart
• Hyperoxia exposure chambers

RECENT REPRESENTATIVE RESEARCH PUBLICATIONS:

• Guidot DM, McCord JM, Wright RM, Repine JE. Absence of electron transport restores growth of a manganese-superoxide dismutase deficient Saccharomyces cerevisiae in hyperoxia: Evidence for electron transport as a major source of superoxide generation in vivo. J Biol Chem 268:26699-26703, 1993.

• Smith D, Kirschenhenter GP, Charlton V, Guidot DM and Repine JE. In vitro selection of RNA-based irreversible inhibitors of human neutrophil elastase. Chemistry and Biology 2:741-750, 1995.

• Wright RM, Vartartes GM, Wiegel LK, Repine JE. Identification of the candidate ALSZ gene at chromosome Zq33 as the human alderhyde oxidase gene. Redor Report 1:313-321, 1995.

• Abraham E, Bursten S, Shenkar R, Allbee J, Tudor R, Woodson P, Guidot DM, Rice G, Singer JW and Repine JE. Phosphatedicacid signaling mediates lung cytokine expression and lung inflammatory injury following hemorrhage. J Exp Med 181:569-575, 1995.

• Guidot DM, Hybertson BM, Kittowski RP and Repine JE. Inhaled NO prevents IL-1 induced neutrophil accumulation and associated acute edema in isolated rat lungs. Am J Physio. 271:225-229, 1996.

• Hybertson BM, Kitlowski RP, Jepson EK and Repine JE: Supercritical fluid-aerosolized vitamin E pretreatment decreases leak in isolated oxidant-perfused rat lungs. J. App. Physio. 84:263-268, 1998.

• Wright RM, Clayton DA, Riley MG, McManaman JL and Repine JE: cDNA cloning, sequencing, and characterization of male and female rat liver aldehyde oxidase (rAOX). Differences in redox status may distinguish male and female forms of hepatic AOX*. J. Biol. Chem. 274:3878-3886, 1999.

• Sharkey RA, Donnelly SC, Connelly KG, Robertson CE, Haslett C and Repine JE: Initial serum ferritin levels in patients with multiple trauma and the subsequent development of acute respiratory distress syndrome. Am. J. Resp. Crit. Care Med. 159:1506-1509, 1999.

LAY SUMMARY OF RESEARCH OBJECTIVES:

To determine the contribution of neutrophil and xanthine oxidase derived O2 metabolites to vascular injury.

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