• General: Macrophage activation and the inflammatory/fibrotic process
• Specific:
• Mechanisms controlling growth factor expression by macrophages
• Intracellular signalling pathways that are activated by TNF-alpha and IFN-gamma and which are integral to the control of growth factor expression
• Investigation of inflammatory gene expression by alveolar macrophages in interstitial lung diseases and cystic fibrosis

• Isolation and culture of macrophages
• Receptor initiated signal transduction
• Control of gene expression
• Recombinant proteins
• Semi-quantitative PCR

• Cottin V, Van Linden A, and Riches DWH. 1999. Phosphorylation of TNF Receptor CD120a (p55) by p42mapk/erk2 Induces Changes in its Subcellular Localization. J. Biol. Chem. 274:32975-32987.

• Uh S-T, Van Linden A, and Riches DWH. 2000. Phosphorylation of 130 and 95 kDa substrates associated with tumor necrosis factor-a receptor CD120a (p55). J. Biol. Chem. 275:793-800.

• Van Linden AA, Cottin V, Leu C, and Riches DWH. 2000. Phosphorylation of the membrane proximal region of TNF receptor CD120a (p55) at ERK consensus sites. J. Biol. Chem. 275:6996-7003.

• Chan ED, Riches DWH, and White C. 2001. Redox paradox: effect of N-acetylcysteine and serum on oxidation-reduction sensitive signaling pathways ERK, p38mapk and JNK Am. J. Respir. Cell. Molec. Biol. 24:627-632.

• Chan ED and Riches DWH. 2001. IFNg and LPS induction of iNOS is modulated by ERK, JNK/SAPK and p38mapk in a mouse macrophage cell line. Am. J. Phyiol. Cell Physiol. 280:C441-C450.

• Cottin V, Van Linden AA, and Riches DWH. 2001. Phosphorylation of TNF receptor CD120a (p55) recruits Bcl-2 and protects against apoptosis. J. Biol. Chem. 276:17252-17260.

• Chan ED, Morris KR, Belisle JT, Hill P, Remigio LK, Brennan PJ and Riches DWH. 2001. Induction of iNOS-NO by lipoarabinomannan of Mycobacterium tuberculosis is mediated by MEK1-ERK, MKK7-JNK and NF-Kb signaling pathways. Infect. Immunity. 69:2001-2010.

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