List of Recent Publications

Aird, W. C., Parvin, J. D., Sharp, P. A., and Rosenberg, R. D. The interaction of GATA-binding proteins and basal transcription factors with GATA box-containing core promoters: a model of tissue specific gene expression. J. Biol. Chem. 269, 883-889 (1994).

Query, C. C., Moore, M. J. and Sharp, P. A. Branch nucleophile selection in pre-mRNA splicing: Evidence for the bulged duplex model. Genes Dev. 8, 587-597 (1994).

Parvin, J. D., Shykind, B. M., Meyers, R. E., Kim, J. and Sharp, P. A. Multiple sets of basal factors initiate transcription by RNA polymerase II. J. Biol. Chem. 269, 18414-18421 (1994).

Sharp, P. A. Split genes and RNA splicing (Nobel Lecture). Cell 77, 805-815 (1994)

Sharp, P. A. Split Genes and RNA Splicing Nobel Lecture, pp. 162-193. 1993 Les Prix Nobel. Published by the Nobel Foundation (1994).

Crispino, J., Blencowe, B. J. and Sharp, P. A. Complementation by SR proteins of pre-mRNA splicing reactions depleted of U1 snRNP. Science 265, 1860-1869 (1994).

Pomerantz, J. L. and Sharp, P. A. Homeodomain determinants of major groove recognition. Biochemistry 33, 10851-10858 (1994).

Blencowe, B. J., Nickerson, J.A., Issner, R., Penman, S. & Sharp, P. A. Association of nuclear matrix antigens with exon-containing splicing complexes. J. Cell Biol. 127, 593-607 (1994).

MacMillan, A. M., Query, C. C., Allerson, C. R., Chen, S., Verdine, G. L., and Sharp, P. A. Dynamic association of proteins with the pre-mRNA branch region. Genes Dev. 8, 3008-3020 (1994).

Zhou, Q. and Sharp, P. A. Novel mechanism and factor for regulation by HIV-1 Tat. EMBO J. 14, 321-328 (1995).

Pomerantz, J. L., Sharp, P. A., and Pabo, C. O. Structure-based design of transcription factors. Science 267, 93-96 (1995).

Kristie, T. M., Pomerantz, J. L., Twomey, T. C., Parent, S. A., and Sharp, P. A. The cellular C1 factor of the herpes simplex virus enhancer complex is a family of polypeptides. J. Biol. Chem. 270, 4387-4394 (1995).

Parvin, J. D., McCormick, R. J., Sharp, P. A., and Fisher, D. E. Pre-bending of a promoter sequence enhances affinity for the TATA-binding factor. Nature 373, 724-727 (1995).

Mäkelä, T. P., Parvin, J. D., Kim, J., Huber, L. J., Sharp, P. A., and Weinberg, R. A. A kinase-deficient transcription factor IIH is functional in basal and activated transcription. Proc. Natl. Acad. Sci. USA 92, 5174-5178 (1995).

Shykind, B., Kim, J., and Sharp, P. A. Activation of the TFIID-TFIIA complex with HMG-2. Genes Dev. 9, 1354-1365 (1995).

Crispino, J. and Sharp, P.A. A U6 snRNA: pre-mRNA interaction can be rate-limiting for U1-independent splicing. Genes Dev. 9, 2314-2323 (1995).

Pomerantz, J. L., Pabo, C. O., and Sharp, P. A. Analysis of homeodomain function by structure-based design of a transcription factor. Proc. Natl. Acad. Sci. USA 92, 9752-9756 (1995).

Blencowe, B. J., Issner, R., Kim, J., McCaw, P., and Sharp, P. A. New proteins related to the Ser-Arg family of splicing factors. RNA 1, 852-865 (1995).

Query, C. C., Strobel, S. A., and Sharp, P. A. Three recognition events at the branch site adenine. EMBO J. 15, 1392-1402 (1996).

Crispino, J. D., Mermoud, J., Lamond, A., and Sharp, P. A. Cis-acting elements from the 5' splice site promote U1-independent pre-mRNA splicing. RNA 2, 664-673 (1996).

Kim, J., Parvin, J.D., Shykind, B.M., and Sharp, P.A. A negative cofactor containing Dr1/p19 modulates transcription with TFIIA in a promoter specific fashion. J. Biol. Chem. 271, 18405-18412 (1996).

Harper, S.E., Qiu, Y., and Sharp, P.A. Sin3 corepressor function in myc-induced transcription and transformation. Proc. Natl. Acad. Sci. 93, 8536-8540 (1996).

Mortillaro, M.J., Blencowe, B.J., Wei, X., Nakayasu, H., Du, L., Warren, S.L., Sharp, P.A. and Berezney, R. A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix. Proc. Natl. Acad. Sci. 93, 8253-8257 (1996).

Cepek, K.L., Chasman, D.I., and Sharp, P.A. Sequence-specific DNA binding of the B-cell-specific coactivator OCA-B. Genes Dev. 10, 2079-2088 (1996).

Zhou, Q. and Sharp, P. A. Tat-SF1: Cofactor for stimulation of transcriptional elongation by HIV-1 Tat. Science. 274, 605-610 (1996).

MacMillan, A. M., McCaw, P. S., Crispino, J.D., and Sharp, P. A. SC35-mediated reconstitution of splicing in U2AF-depleted nuclear extract. Proc. Natl. Acad. Sci. USA 94, 133-136 (1997).

Shykind, B. M., Kim, J., Stewart, L., Champoux, J. and Sharp, P.A. Topoisomerase I enhances TFIID-TFIIA complex assembly during activation of transcription. Genes Dev. 11, 397-407 (1997).

Kim, J.-S., Kim, J., Cepek, K.L., Sharp, P.A., and Pabo, C.O. Design of TATA-box binding protein/zinc finger fusions for targeted regulation of gene expression. Proc. Natl. Acad. Sci. 94, 3616-3620 (1997).

Query, C. C., McCaw, P. S. and Sharp, P.A. A minimal spliceosomal A complex recognizes branch site and polypyrimidine tracts. Mol. Cell. Biol. 17, 2944-2953 (1997).

Jones, G. and Sharp, P.A. Ultraspiracle: an invertebrate nuclear receptor for juvenile hormone. Proc. Natl. Acad. Sci. 94, 13499-13503 (1997).

Sharp, P. A. and Burge, C. B. Classification of introns: U2-type or U12-type. Cell (Minireview) 91, 875-879 (1997).

Blencowe, B.J., Issner, R., Nickerson, J., and Sharp, P.A. A coactivator of pre-mRNA splicing. Genes Dev. 12, 996-1009 (1998).

Tuschl, T., Sharp, P.A., and Bartel, D.P. Selection in vitro of novel ribozymes from a partially randomized U2 and U6 snRNA library. EMBO J. 17, 2637-2650 (1998).

Lim, L. P. and Sharp, P. A. Altenative splicing of the fibronectin EIIIB exon depends on specific TGCATG repeats. Mol. Cell. Biol. 18, 3900-3906 (1998).

Burge, C., Padgett, R., and Sharp, P.A. Evolutionary fates and origins of U12-type introns. Mol. Cell 2, 773-785 (1998).

Burge, C., Tuschl, T. and Sharp, P.A. Splicing of precursors to mRNAs by the spliceosomes. In RNA World II, R. Gesteland, T. Cech, and J. Atkins, eds., Cold Spring Harbor Laboratory Press, NY. pp. 525-560 (1999).

Sharp, P.A. RNAi and double strand RNA. Genes Dev. (Perspective) 13, 139-141 (1999).

Eldridge, A., Li, Y., Sharp, P. A., and Blencowe, B. The SRm160/300 splicing coactivator is required for exon-enhancer function. Proc. Natl. Acad. Sci. 96, 6125-6130(1999).

Mitsui, A. and Sharp, P.A. Ubiquitination of RNA polymerase II large subunit signaled by phosphorylation of carboxyl-terminal domain. Proc. Natl. Acad. Sci. 96, 6054-6059 (1999).

Kim, J.B., Yamaguchi, Y., Wada, T., Handa, H., and Sharp, P.A. Tat-SF1 protein associates with RAP30 and hSPT5 proteins. Mol. Cell Biol. 19, 5960-5968 (1999).

Tang, H. and Sharp, P.A. Transcriptional regulation of the murine 3' IgH enhancer by phosphorylated Oct-2. Immunity 11, 517-526 (1999).

Gilbert, S. and Sharp, P.A. Targeted deacetylation of promoter regions of X-inactivated genes. Proc. Natl. Acad. Sci 96, 13825-13830 (1999).

Tuschl, T., Zamore, P.D., Lehmann, R., Bartel, D.P. and Sharp, P.A. Targeted mRNA degradation by double-stranded RNA in vitro. Genes Dev. 13, 3191-3197 (1999).

McCaw, P., Amonlirdviman, K., and Sharp, P.A. A novel U2AF65-related splicing activity. RNA 5, 1548-1560 (1999).

Chasman, D., Cepek, K., Sharp, P.A., and Pabo, C.O. Crystal structure of a ternary complex containing an OCA-B peptide, Oct-1, and an octamer element: Peptide recognition of a protein-DNA interface. Genes Dev. 13, 2650-2657 (1999).

Blencowe, B.J., Baurén, G., Issner, R., Eldridge, A., Li, Y., Reifenberg, E., and Sharp, P.A. The SRm160/300 splicing coactivator subunits. RNA 6, 111-120 (1999).

Scarr, R., Smith, M., Beddall, M., and Sharp, P.A. A unique 50 kD form of HCF found in Go cells. Mol. Cell. Biol., in press (2000).

Tuschl, T., Sharp, P.A. and Bartel, D.P. A ribozyme selected from variants of U6 snRNA promotes 2',5'-branch formation. Submitted (2000).

Zamore, P.D., Tuschl T. , Sharp, P.A., and Bartel, D.P. RNAi: dsRNA Directs the ATP-Dependent Cleavage of mRNA at 21 to 23 Nucleotide Intervals. Cell, in press (2000).

Sharp, P.A. and Zamore, P.D. RNA interference (RNAi). Science perspective, in press (2000).

Gilbert, S., Pehrson, J. and Sharp, P.A. Xist RNA is a component of the inactive X chromatin. In preparation (1999).