Resources
Transgenic and Gene Targeting Core
Message from the Director:
A new "Transgenic and Gene Targeting Core" has been established in the Charles C. Gates Regenerative Medicine and Stem Cell Biology Program at UCD. Our core facility replaces the Transgenic/Knockout Core that was part of the UCD Cancer Center. We are committed to provide the UCD research community with state-of-the-art services. Our goal is to assist PIs in designing and generating genetically engineered mouse lines to further biomedical research.
Our services include pronuclear injections (conventional and BAC transgenic mice), ES cell injections (e.g. knockout and knockin mice), cryo-preservation of embryos and embryo re-derivation (see below for a detailed
list of services and prices). We also conduct gene targeting experiments. The PIs will provide the gene targeting construct and test ES cell clones for homologous recombination. We will conduct the entire cell culture work and inject recombinant ES cell clones into mouse embryos to generate chimeric mice. If requested, we will breed these chimeras to test for germline transmission of mutant alleles.
We also provide consultation services for the UCD community. Usually, we discuss experimental design before we actually initiate the project. The goal is to ensure that the experimental strategy employed is likely to result in the successful generation of the desired mouse line. The initial consultation (usually a one hour meeting) is provided free of charge. Further consultations and assistance in designing the experimental strategy to generate and analyze mouse lines will be provided on a fee-for-service basis.
To streamline our communication with PIs, we have established an e-mail account that should be used to schedule services and to request consultations (makeamouse@ucdenver.edu). All core members have access to this e-mail account. To facilitate correct routing of your requests, please include the PI name and the requested service in the header of your e-mail (e.g. John Doe “schedule transgene injection"; John Doe “schedule consultation"). We will then route the request to the appropriate core member.
We are planning to introduce new services to accommodate the needs of our research community. This might include the generation of transgenic rats, and the use of retroviral vectors to generate transgenic mice. We will evaluate requests for services from the research community for new services on a regular basis.
We are also planning to provide you with transgene and gene targeting vector design and construction services in the near future. Please stay tuned. I will need your feedback with respect to the introduction of new services. Feel free to drop me an e-mail at makeamouse@ucdenver.edu
Lastly, our core services are partially supported by a grant from the SOM dean. We are planning to become self-sufficient within three years. This will not be possible without your support. Please use our services and provide us with feedback so that we can continue to improve our services and help you to achieve your research goals.
Peter J. Koch
Core Personnel:
- Peter J. Koch, Ph.D.
- Associate Professor of Dermatology
Director, Transgenic and Gene Targeting Core
Charles C. Gates Regenerative Medicine and Stem Cell Biology Program
University of Colorado Denver
(P) 303 724 0051
E-Mail: Peter.Koch@ucdenver.edu - Joseph Anderson, M.S., Laboratory Manager
- Senior PRA
Embryo Manipulation Services
E-Mail: Joseph.Anderson@ucdenver.edu - Ling Wang, M.D.
- PRA
Gene targeting in ES cells
E-Mail: Ling.Wang@ucdenver.edu - Wallace Chick, Ph.D.
- Instructor
Transgene and Gene Targeting Vector Design
E-Mail: Wallace.Chick@ucdenver.edu - Shawna Johnson, B.S.
- PRA
Breeding and maintenance of genetically engineered mouse lines
E-Mail: Shawna.Johnson@ucdenver.edu - Abby Knight, CVT, RLAT
- PRA
Colony and Data Management
E-Mail: Abby.Knight@ucdenver.edu
To request or schedule services please send an e-mail to:
MAKEAMOUSE@ucdenver.edu
Please
include the PI name and the type of service requested in the subject line.
Information and Protocols:
Producing Fertile Embryos for Cryopreservation and Rederivation